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MEDICINA DI LABORATORIO E DIAGNOSTICA INTEGRATA - channel 3

Teaching Staff

LIVIA MANZELLA - Module Clinical Pathology - MED/05 - 2 CFU
VITTORIO CALABRESE - Module Clinical Biochemistry - BIO/12 - 2 CFU
GUIDO SCALIA - Module Microbiology and Clinical Microbiology - MED/07 - 2 CFU

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Learning Objectives

• Clinical Pathology
  

  The course develops skills in the Clinical Pathology aiming to teach the student to apply, evaluate and interpret the laboratory tests used to study of the main diseases of the blood, alterations of the coagulative-fibrinolytic system, changes in liver and kidney function, some endocrinopathies, dyslipidemia, myocardial infarction, autoimmune diseases and cancer.

• Clinical Biochemistry
  

  The course is designed to provide students with a good foundation of knowledge in all aspects of Clinical Biochemistry. Introducing the fundamentals of instrumentation and methodology in the clinical chemistry and biochemistry laboratory medicine, lectures will cover urinalysis testing procedures and associated disease entities, as well as analysis of other body fluids. Clinical testing using automated and manual methods, measurement of pancreatic function and intestinal absorption, renal and liver function, enzymes, electrolytes, blood gases, lipids, toxicology, urinalysis, endocrinology, neurological, dysmetabolic, inflammatory and cardiovascular diseases.

• Microbiology and Clinical Microbiology
  

  Knowledge of prevention and prophylaxis criteria of infections and possible therapeutic opportunities; pathology and pathogenesis of the main infectious agents involved in human pathology; paths of follow-up, diagnosis, and therapy; apply the knowledge of microbiology to human disease by systems or organs.

Course Structure

• Clinical Biochemistry
  

  Frontal teaching, elaboration, Individual study

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Detailed Course Content

• Clinical Pathology
  

  Laboratory analysis: definition, typology

  Collection and conservation of biological samples

  Preanalytical, analytical and biological variability

  Complete blood count test

  Plasma proteins and electrophoresis

  Hemostasis test

  Blood groups and compatibility testing

  Urine analysis and functional kidney test

  The functional liver test

  Diabetes: diagnosis and follow up test

  Myocardial Infraction test

  Autoimmune disease test

  Cerebrospinal liquor

  Tumoral Markers

• Clinical Biochemistry
  

  1) The use of laboratory data in clinical practice
  2) The method of acquisition of laboratory data
  3) The interpretation of biochemical data
  4) Analysis of Urine
  5) Biochemistry of nutrition
  6) Assessment of liver function and diagnosis of jaundice
  7) Acute and chronic diseases of the liver
  8) Liver function and Hepatitis tests
  9) Diabetes mellitus
  10) Hypoglycemia
  11) Lipid metabolism, dyslipidemia and atherosclerosis, sphingolipidoses.
  12) Free radicals in the pathology
  13) Metabolism of ethanol and the pathology of alcoholism
  14) Complete blood count test
  15) Disorders of porphyrin and heme synthesis, hemoglobinopathies
  16) Plasma proteins and electrophoresis
  17) Blood groups and compatibility tests
  18) Hemostasis tests
  19) Kidney function tests and urine test
  20) Iperammoniemie;
  21) Metabolism of amino acids (Phenylketonuria, Hyperhomocysteinemia);
  22) Disorders salt and water balance and acid-base balance;
  23) Disorders of purine metabolism (hyperuricemia, gout)
  24) Investigations on the cerebrospinal fluid
  25) Biochemical aspects of neurological diseases
  26) Tumor markers
  27) Metabolic effects of tumors
  28) Cellular aspects of clinical biochemistry
  29) Molecular Clinical Biochemistry
  30) Clinical biochemistry in the diagnosis of acute pain in the chest and abdomen
  31) Biochemistry and molecular clinical biology of organ transplantation
  32) Notes on antiaging medicine.

• Microbiology and Clinical Microbiology
  

  GENERAL PRINCIPLES of Clinical Microbiology: microbial ecology; pathogenicity and virulence; infection and disease; epidemiology of infectious diseases; nosocomial infections.

  METHODS in Clinical Microbiology: Clinical Question and requests for investigation; direct and indirect diagnosis; sampling; elements of microbiological techniques; interpretation of the response.

  CLINICAL MICROBIOLOGY: Aetiological agent, symptoms, clinical sample, prevention, prophylaxis, therapy, and diagnosis of infections of:

  upper respiratory tract; lower respiratory tract; cardiovascular apparatus; gastrointestinal apparatus; hematopoietic apparatus; central nervous system; sexually transmitted diseases; urogenital apparatus; eye; ear, nose and throat; skin and mucous membranes; wound infections; bones and articulations; immunocompromised host infection; TORCH; diagnosis prevention and follow-up of HPV infections.

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Textbook Information

• Clinical Pathology
  

  1. Sacchetti L., Cavalcanti P. et. al. Medicina di laboratorio e diagnostica genetica. Sorbona

  2. Laposata M. Medicina di Laboratorio. Piccin

  3. Antonozzi I. Medicina di Laboratorio. Piccin

  4. Federici G. Medicina di Laboratorio. Mc Graw-Hill Companies

  5. Balestrieri et al. Diagnostica Molecolare nella Medicina di Laboratorio. Piccin

• Clinical Biochemistry
  

  Medicina di Laboratorio, G. Federici (Autore), – Mc GrawHill Medicina di laboratorio.

  Medicina di Laboratorio. La diagnosi di malattia nel laboratorio clinico, M. Laposata (Autore) - Piccin

  Medicina di Laboratorio. Logica e patologia clinica, I. Antonozzi, E. Gulletta (Autori) - Piccin

  Medicina dell'Aging e dell'Antiaging. V. Calabrese et al. (Autori) - Edra

  Nutrigenomica e Epigenetica. V. Calabrese et al. (Autori) - Edra

• Microbiology and Clinical Microbiology
  
  1. Antonelli, Clementi, Pozzi, Rossolini : Principi di Microbiologia medica – Ed. Ambrosiana
  2. La Placa : Principi di Microbiologia Medica – Ed. Esculapio
  3. Jawetz, Melnick, Adelberg’s: Microbiologia medica – Ed. Piccin
  4. Sherris: Microbiologia Clinica, Ed. EMSI

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