MICROBIOLOGIA E MICROBIOLOGIA CLINICA - III ANNO

Critically analyze and describe, for each "microbial species": the ways of penetration into the organism, the differentiated diffusion in the infected host, the presence of antigens in the various parts of the organism (blood, secretions, excretions) for the purpose of "contagion "And laboratory diagnosis. Monitor the epidemiology of infectious diseases (public health microbiology)

Frontal oral presentation. Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus

1. The Clinical Microbiology laboratory and the main diagnostic procedures in microbiology
   1. The management of the laboratory
   2. Pre-analytical phase
   3. Analytical phase
   4. The main diagnostic procedures in microbiology
      1. the. Cultivation techniques
         1. Bacteria
         2. Viruses
         3. Fungi
      2. Independent culture techniques
         1. Histological techniques
         2. Molecular techniques
            1. Target amplification
            2. Amplification of the "probes"
            3. Signal amplification
            4. Hybridization
            5. Sequencing systems
            6. The application of omics in clinical microbiology
               1. Microbiomics
               2. Metagenomics
               3. Metabolomics
               4. iv. Other "omics" sciences
            7. Proteomics techniques (MALDI-TOF etc.)
            8. Chromatographic techniques
            9. Other methodologies
         3. Serological techniques
            1. Immunofluorescence
            2. Immunoenzymatic
            3. Immunochromatographic systems
   5. Quality control in the clinical microbiology laboratory
      1. ISO and UNI EN procedures
2. Diagnostic models applied to organ and system infectionsThe ways of penetration into the body, the differentiated diffusion in the infected host, the presence of antigens in the various parts of the organism (blood, secretions, excretions) for the purpose of "contagion" and laboratory diagnosis of
   1. Main pathogenic bacteria for humans
      1. Staphylococcus, Streptococcus, Enterococcus, Neisseria, Branhamella, Mycobacterium, Streptomyces, Nocardia, Actinomyces, Corynebacterium, Lactobacillus, Bartonella, Listeria, Gardnerella, Bacillus, Clostridium, Enterobacteriaceae, Haemurophilus, Legucacterella, Legucacterella, Pas- monophilus Bacteroides, Campylobacter, Helicobacter, Mycoplasma, Ureaplasma, Chlamydiaceae, Rickettsiales, Spirochetales
   2. Main viruses responsible for human infections
      1. Poxviridae, Herpesviridae, Adenoviridae, Papillomaviridae, Polyomaviridae (Human), Parvoviridae, Paramyxoviridae, Pneumoviridae, Orthomyxoviridae, Picornaviridae, Arenaviridae, Peribunyaviridae, Hantaviridae, Nairoviridae, Caliciviridae, Coronaviridae, Filoviridae, Flaviridae, Reoviridae, Retroviridae (human), Togaviridae, Matonaviridae (Rubivirus), Rhabdoviridae (Rabies lyssavirus), hepatitis virus (HAV, HBV, HCV, HDV, HEV, HGV).
   3. Main fungi responsible for infections in humans
      1. Primary dimorphic pathogens responsible for deep mycosis:
         1. Histoplasma capsulatum var. capsulatum, Histoplasma capsulatum var. duboisii, Blastomyces dermatitidis, Coccidioides immits, Paracoccidioides brasiliensis,
      2. Dimorphic opportunistic pathogens responsible for deep mycosis:
         1. Talaromyces marneffei
      3. Superficial mycoses:
         1. Aspergillus spp., Rhizopus spp., Scopulariopsis spp., Mucor spp., Penicillium spp, Malassezia furfur, Exophiala werneckii, Trichosporon beigelii, Piedraia hortae, Candida spp.
      4. Cutaneous mycoses.
         1. Dermatophytosis:
         2. to. Microsporum spp .., Trichophyton spp. , Epidermophyton spp ..
      5. Dermatomycosis:
         1. Hendersonula toruloidea, Scytalidium hyalium (Natrassia spp.), Scopulariopsis brevicaulis.
      6. Subcutaneous mycoses (outline).
         1. Sporotrichosis: Sporothrix spp.
         2. Chromoblastomycosis: Fonsecaea, Phialophora, Cladophialophora.
         3. Feoifomycosis: Cladophialophora spp, Exophiala spp, Bipolaris spp, Exserohilum spp.
         4. Fungal mycetoma: Scedosporium spp, Madurella spp, Trematosphaeria spp, Acremonium spp, Exophiala spp.
         5. Entomophtoromycosis: Basidiobolus ranarum, Conidiobolus coronatus.
         6. Mucormycosis: Rhizopus spp, Mucor spp, Rhizomucor spp, Lichtheimia spp, Saksenaea spp. Lobomycosis: Loboa loboi. Rhinosporidiosis: Rhinosporidium seeberi.
      7. Systemic mycoses due to opportunistic fungi:
         1. Candidiasis: Candida spp, Debaryomyces spp, Kluyveromyces spp, Meyerozyma spp, Pichia spp .. Cryptococcosis: Cryptococcus spp, C. neoformans / C. catsi.
         2. Aspergillosis: Aspergillus fumigatus complex, A. flavus, complex, A. terreus complex. Scedosporiosis (notes): Scedosporium spp., Lomentospora spp.
         3. Mucormycosis: Rhizopus spp, Mucor spp, Rhizomucor spp, Lichtheimia spp.
         4. Ialoifomycosis: Penicillium spp, Paecilomyces spp, Beauveria spp, Fusarium spp., Scopulariopsis spp.
         5. Phaeohyphomycosis (outline): Cladophialophora spp, Exophiala spp, Bipolaris spp, Exserohilum spp
      8. Notes on Microsporidiosis and Pneumocystis spp
3. Public health microbiology
   1. The epidemiology of infectious diseases
   2. General concepts
   3. Epidemiological terminology
   4. Frequency indexes
   5. is. Recognition of an infectious disease within a population
   6. Recognition of an epidemic
   7. The infectious cycle: history of a disease
   8. Carriers and reservoirs
   9. the. Health surveillance and the role of the clinical microbiologist
4. New frontiers of clinical microbiology: the medico-legal aspects of clinical microbiology and forensic microbiology
   1. Medico-legal aspects of laboratory tests
   2. Food infections and toxic infections and failure to follow self-control procedures
   3. Clinical microbiology and virology in the context of autopsy
   4. Medico-legal implications for hospital infections
   5. Bioterrorism and Biocontainment
   6. Legislative Decree 81/2008 and its amendments: microbiological aspects of biocontainment and the organization of the Clinical Microbiology laboratory

Topley & Wilsons' su: https://onlinelibrary.wiley.com/doi/book/10.1002/9780470688 (il link funziona esclusivamente dentro la rete dell'Ateneo di Catania)

Koneman's - Color Atlas and textbook of Diagnostic Microbiology 7ed. Wolters Kluver - International Edition ISBN-13: 978-1-4511-8935-3