BIOLOGIA MOLECOLARE AVANZATA

The Molecular Biology Advanced class is aimed to enlarge the knowledge on the topics carried out in the Molecular Biology and Bioinformatic class (2^nd year, 1^st semester).

A clear perspective of the molecular mechanisms underneath the regulation of human genome transcription and translation, coupled with the deep understanding of the role played by microRNA and long-noncoding RNA in the regulation of the main biological functions, will be provided. Students will be able to link molecular structures with their relative molecular functions, and therefore with complex biological pathways.

Knowledge regarding up-to-date molecular tools such as next-generation sequencing or CRISPR gene editing will be also taught to students.

Lectures will be performed, with the support of ppt slide-decks, which will be available for the students.

Frequency is mandatory as defined by CdS regulations.

Pre-existing knowledge of Molecular Biology and General Biochemistry is compulsory.

Regulation of the transcription in the human genome.

Study of proteins-nucleic acids interactions.

Regulation of the translation: miRNA (other small RNAs) e long ncRNAs.

Proteins processing and post-translational modifications.

DNA recombination and repair mechanisms.

Advanced molecular biology techniques: tg mice, KO mice, next-generation sequencing e CRISPR gene editing.

Zlatanova & van Holde, Biologia Molecolare: Struttura e dinamica dei Genomi e Proteomi, Zanichelli 2018 (ITA).

The textbook will be integrated with papers and reviews to cover the up-to-date knowledge on topics not covered by the book or which need deepen analysis (ENG).