1013130 - METODOLOGIE DIAGNOSTICHE DI BIOCHIMICA CLINICA E DI BIOLOGIA MOLECOLARE CLINICA
Module 1013132 - METODOLOGIA DIAGNOSTICA MOLECOLARE
Expected Learning Outcomes
The specific training objectives of the Laboratory Diagnostic Methodologies Course are to enable students to acquire the basic notions for critical evaluation and interpretation of the results of the main diagnostic and biomolecular techniques used in clinical practice and basic research.
Course Structure
The teaching will be carried out through the provision of classroom teaching lessons plus laboratory exercises to be carried out in the laboratories of the Biological Tower in order to improve the theoretical and practical knowledge acquired during the hours spent in the classroom.
Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
Required Prerequisites
Basic knowledge of diagnostic and molecular biology methodologies.
Attendance of Lessons
Detailed Course Content
First generation sequencing: Sanger and Pyrosequencing
Microarray of gene expression
New generation sequencing NGS - Illumina and Roche 454 technology
Digital PCR - Droplet Digital PCR
Consultation of scientific literature and design of primers for PCR
Textbook Information
Widmann: Interpretazione clinica degli esami di laboratorio. Ronald A. Sacher e Richard A. McPherson. 2001 Undicesima edizione. Mc Graw Hill libri Italia (Milano)
Giorgio Federici: Medicina di Laboratorio. 2008 Terza edizione. Mc Graw Hill libri Italia (Milano)
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | Sequenziamento di prima generazione: Metodo Sanger | Professor-made materials |
| 2 | Pirosequenziamento | Professor-made materials |
| 3 | Sequenziamento di nuova generazione NGS: Tecnologia Illumina e Roche 454 | Professor-made materials |
| 4 | Digital PCR | Professor-made materials |
| 5 | Droplet Digital PCR | Professor-made materials |
Learning Assessment
Learning Assessment Procedures
The learning assessment consists of an oral test on the contents indicated in the program.
Learning assessment may also be carried out on line, should the conditions require it.
Examples of frequently asked questions and / or exercises
Differences between the first generation sequencing and the next generation sequencing.
Workflow of the droplet digital PCR
Versione in italiano