SCIENZE MEDICHE, CHIRURGICHE E TECNOLOGIE AVANZATE G.F. INGRASSIABiomedical Laboratory techniquesAcademic Year 2022/2023
1007347 - METODOLOGIE DIAGNOSTICHE DI ANATOMIA PATOLOGICA
Module 1007350 - TECNICHE DI IMMUNOISTOCHIMICA E PATOLOGIA MOLECOLARE
Teacher: Gaetano Giuseppe MAGRO
Expected Learning Outcomes
Knowledge of the main immunohistochemical and biomolecular techniques.
Course Structure
Frontal teaching.
Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
Detailed Course Content
Enzymatic histochemistry (special stains: eg Alcian-PAS, Giemsa, Rosso Congo, etc.)
immunohistochemistry: unmasking, detection systems, antigen-antibody reaction, primary and secondary antibodies
Main molecular biology techniques (FISH, CISH)
Textbook Information
Ruco Scarpa- Anatomia Patologica- Le basi, UTET
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | Istochimica enzimatica (colorazioni speciali: es. Alcian-PAS, Giemsa, Rosso Congo,ecc) | Ruco-Scarpa Anatomia Patologica – Le Basi UTET |
| 2 | immunoistochimica: smascheramento, sistemi di rivelazione, reazione antigene-anticorpo, anticorpi primari e secondari | Ruco-Scarpa Anatomia Patologica – Le Basi UTET |
| 3 | Principali tecniche di biologia molecolare (FISH, CISH) | Ruco-Scarpa Anatomia Patologica – Le Basi UTET |
Versione in italiano