Master's Degree in Medical Biotechnologies
Academic Year 2018/2019 - 2° Year
PATOLOGIA GENERALE E METODOLOGIE DIAGNOSTICHE DI LABORATORIO
Teaching Staff
LUCIA MALAGUARNERA - Module PATOLOGIA GENERALE - MED/04 - 3 CFUSALVATORE TRAVALI - Module ONCOLOGIA MOLECOLARE - MED/04 - 3 CFU
MASSIMO LIBRA - Module LABORATORY DIAGNOSTICS METHODS - MED/46 - 4 CFU
Learning Objectives
- LABORATORY DIAGNOSTICS METHODS
The specific training objectives of the Laboratory Diagnostic Methodologies Course are to enable students to acquire the basic notions for critical evaluation and interpretation of the results of the main diagnostic and biomolecular techniques used in clinical practice and basic research.
Course Structure
- LABORATORY DIAGNOSTICS METHODS
The teaching will be carried out through the provision of classroom teaching lessons plus laboratory exercises to be carried out in the laboratories of the Biological Tower in order to improve the theoretical and practical knowledge acquired during the hours spent in the classroom.
Detailed Course Content
- LABORATORY DIAGNOSTICS METHODS
1. First generation sequencing: Sanger and Pyrosequencing method.
2. Microarray of gene expression.
3. New generation sequencing NGS - Illumina technology pt 1/2
4. New generation NGS sequencing - Illumina pt 2/2 and Roche 454 technology
5. Droplet Digital PCR.
6. Role of the Microbiota and its implications in oncological and neuropsychiatric pathologies
7. Consultation of scientific literature and design of primers for PCR.
Textbook Information
- LABORATORY DIAGNOSTICS METHODS
1) Widmann: Interpretazione clinica degli esami di laboratorio. Ronald A. Sacher e Richard A. McPherson. 2001 Undicesima edizione. Mc Graw Hill libri Italia (Milano)
2) Giorgio Federici: Medicina di Laboratorio. 2008 Terza edizione. Mc Graw Hill libri Italia (Milano)
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