BIOCHIMICA E BIOLOGIA MOLECOLARE

10 CFU - 1° Semester

Teaching Staff

ROBERTO AVOLA - Module Biochemistry - BIO/10 - 4 CFU
VITO NICOLA DE PINTO - Module BIOLOGIA MOLECOLARE - BIO/11 - 2 CFU
VINCENZA BARRESI - Module Clinical Biochemistry and Clinical Molecular Biology (2 CFU) - BIO/12 - 2 CFU
VINCENZA BARRESI - Module Clinical Biochemistry and Clinical Molecular Biology (2 CFU), 2nd year, 1st semester - BIO/12 - 2 CFU

Learning Objectives

Biochemistry
The course of Biochemistry aims to provide to the students the basis to understanding the physical, chemical and biological contexts in which molecules, reactions and metabolic pathways play their role. Particular attention will be given to the structure and function relationship of the major classes of macromolecules as also to the metabolic regulation at the molecular and cellular level. In order to stimulate student interest, the topics will be explained emphasizing the logical and consequential interconnections emphasizing the clinical aspects and introducing experimental methods. At the end of the present course, the student will understand the structure-function relationships of the main biological molecules, essential biochemical mechanisms that underlie a proper metabolic function and the consequences of their alterations.
BIOLOGIA MOLECOLARE
The course of Biochemistry and Molecular biology aims to provide to the students the basis to understanding the physical, chemical and biological contexts in which molecules, reactions and metabolic pathways play their role. Particular attention will be given to the structure and function relationship of the major classes of macromolecules as also to the metabolic regulation at the molecular and cellular level. In order to stimulate student interest the topics will be explained emphasizing the logical and consequential interconnections emphasizing the clinical aspects and introducing experimental methods. At the end of the present course the student will understand the structure-function relationships of the main biological molecules, essential biochemical mechanisms that underlie a proper metabolic function and the consequences of their alterations.
Clinical Biochemistry and Clinical Molecular Biology (2 CFU)
Biochemical and molecular alterations, biological samples and molecular tests in clinical biochemistry and molecular biology with particular relevance to the techniques to detect the physiopathology markers of the oral cavity
Clinical Biochemistry and Clinical Molecular Biology (2 CFU), 2nd year, 1st semester
Biochemical and molecular alterations, biological samples and molecular tests in Clinical Biochemistry and molecular biology Laboratories with particular relevance to the metabolic physiopathology of the oral cavity

Detailed Course Content

Clinical Biochemistry and Clinical Molecular Biology (2 CFU)
Biological samples, extraction and dosage of nucleic acids and proteins.

Molecular Biology Techniques in the Laboratory: Chain Polymerization Reaction (PCR), PCR applied to detection of oral cavity markers

Enzyme immunoassays (ELISA)

Sequencing I, II, III Material provided during the course by the teacher

Microarrays technologies

Tumor markers, tumors of the oral cavity
Clinical Biochemistry and Clinical Molecular Biology (2 CFU), 2nd year, 1st semester
Definition and role of laboratory medicine, laboratory investigation

Biological samples: types, sampling, processing and storage. Extraction and assay of nucleic acids.

The biological report: preanalytical variability, analytical and post-analytical. biological variability. Quality control.

Diagnostic characteristics of a laboratory test: diagnostic sensitivity, diagnostic specificity, positive predictive value and negative predictive test.

Liver, metabolism, markers of liver injury, detoxification reactions, alcoholism.

Heart, markers of cardiac damage, Investigations.

Cardiovascular risk: atherosclerosis and lipid metabolism, laboratory investigations. Kidney: Parameters for assessment of renal function and urine analysis

Complete Blood Count (CBC): Results and Interpretation

Calcium & Phosphorus Metabolism

Metabolism of the oral cavity, caries.

Textbook Information

Biochemistry
1. Siliprandi-Tettamanti. Biochimica Medica. Piccin.
2. Nelson Cox. I principi di Biochimica di Lehninger. Zanichelli
Clinical Biochemistry and Clinical Molecular Biology (2 CFU)
1)Material provided during the course by the teacher1)Medicina di Laboratorio e Diagnostica Genetica, L. Sacchetti, et al. Idelson-Gnocchi. 2)Medicina di Laboratorio, M. Zatti, et al., Idelson-Gnocchi. 3)Biologia del cavo orale. Istologia, biochimica, fisiologia, David B. Ferguson, CE.
Clinical Biochemistry and Clinical Molecular Biology (2 CFU), 2nd year, 1st semester
1) Additional material supplied by the teacher 2)Onlines courses; 3)Medicina di Laboratorio e Diagnostica Genetica, L. Sacchetti, et al. Idelson-Gnocchi. 2)Medicina di Laboratorio, M. Zatti, et al., Idelson-Gnocchi. 4)Biologia del cavo orale. Istologia, biochimica, fisiologia, David B. Ferguson, CEA; 5)Biochimica medica strutturale metabolica e funzionale, Noris Siliprandi,Guido Tettamanti, Piccin-Nuova Libreria

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