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MEDICINA DI LABORATORIO E DIAGNOSTICA INTEGRATA - channel 1

Teaching Staff

LIVIA MANZELLA - Module Clinical Pathology - MED/05 - 2 CFU
VITTORIO CALABRESE - Module Clinical Biochemistry - BIO/12 - 2 CFU
MARIO SALMERI - Module Clinical microbiology - MED/07 - 2 CFU

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Learning Objectives

• Clinical Pathology
  

  The course develops skills in the Clinical Pathology aiming to teach the student to apply, evaluate and interpret the laboratory tests used to study of the main diseases of the blood, alterations of the coagulative-fibrinolytic system, changes in liver and kidney function, some endocrinopathies, dyslipidemia, myocardial infarction, autoimmune diseases and cancer.

• Clinical Biochemistry
  

  The course is designed to provide students with a good foundation of knowledge in all aspects of Clinical Biochemistry. Introducing the fundamentals of instrumentation and methodology in the clinical chemistry and biochemistry laboratory medicine, lectures will cover urinalysis testing procedures and associated disease entities, as well as analysis of other body fluids. Clinical testing using automated and manual methods, measurement of pancreatic function and intestinal absorption, renal and liver function, enzymes, electrolytes, blood gases, lipids, toxicology, urinalysis, endocrinology, neurological, dysmetabolic, inflammatory and cardiovascular diseases.

• Clinical microbiology
  

  Knowledge of microbiological and virological diagnosis

  • Knowledge the tests used in microbiological and virological diagnosis

  • Biological samples suitable for microbiological and virological diagnosis

  • Specimen collection and storage of samples used for diagnostic purposes

  • Interpretation of the results of microbiological tests

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Detailed Course Content

• Clinical Pathology
  

  Laboratory analysis: definition, typology

  Collection and conservation of biological samples

  Preanalytical, analytical and biological variability

  Complete blood count test

  Plasma proteins and electrophoresis

  Hemostasis test

  Blood groups and compatibility testing

  Urine analysis and functional kidney test

  The functional liver test

  Diabetes: diagnosis and follow up test

  Myocardial Infraction test

  Autoimmune disease test

  Cerebrospinal liquor

  Tumoral Markers

• Clinical Biochemistry
  

  1) The use of laboratory data in clinical practice
  2) The method of acquisition of laboratory data
  3) The interpretation of biochemical data
  4) Analysis of Urine
  5) Biochemistry of nutrition
  6) Assessment of liver function and diagnosis of jaundice
  7) Acute and chronic diseases of the liver
  8) Liver function and Hepatitis tests
  9) Diabetes mellitus
  10) Hypoglycemia
  11) Lipid metabolism, dyslipidemia and atherosclerosis, sphingolipidoses.
  12) Free radicals in the pathology
  13) Metabolism of ethanol and the pathology of alcoholism
  14) Complete blood count test
  15) Disorders of porphyrin and heme synthesis, hemoglobinopathies
  16) Plasma proteins and electrophoresis
  17) Blood groups and compatibility tests
  18) Hemostasis tests
  19) Kidney function tests and urine test
  20) Iperammoniemie;
  21) Metabolism of amino acids (Phenylketonuria, Hyperhomocysteinemia);
  22) Disorders salt and water balance and acid-base balance;
  23) Disorders of purine metabolism (hyperuricemia, gout)
  24) Investigations on the cerebrospinal fluid
  25) Biochemical aspects of neurological diseases
  26) Tumor markers
  27) Metabolic effects of tumors
  28) Cellular aspects of clinical biochemistry
  29) Molecular Clinical Biochemistry
  30) Clinical biochemistry in the diagnosis of acute pain in the chest and abdomen
  31) Biochemistry and molecular clinical biology of organ transplantation
  32) Notes on antiaging medicine.

• Clinical microbiology
  

  • The micro-organisms which cause infection and disease: concept of pathogenicity and virulence, host situations predisposing to infections

  • Elements of Microbiological Diagnosis: direct and indirect tests: isolation of micro-organisms and viruses. detection and dosage of antibodies

  • Choosing of the most suitable samples for diagnosis.

  • Principles, aims and interpretation of the Antibiogramma. MIC, MBC.

  • Elements of Virological Diagnosis: direct and indirect tests. Viral isolation, selection of most suitable samples and methods to preserve and keep them

  • infections of the eye and laboratory diagnosis methods

  • Infections of the CNS and laboratory diagnosis methods

  • Infections of the upper and lower respiratory tract and diagnostic considerations.

  • Infections of the cardiovascular and laboratory diagnosis methods

  • Diagnosis of infectious gastroenteritis and toxic food infections

  • diagnosis of urinary tract infections • Infections in pregnancy: diagnosis of infection from the mother

  • Sexually transmitted infections and laboratory diagnosis method

  • infection of the skin, soft tissue and laboratory diagnosis methods

  • Microbiological control of hospital infections

  • infections in the immunocompromised patient

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Textbook Information

• Clinical Pathology
  

  1. Sacchetti L., Cavalcanti P. et. al. Medicina di laboratorio e diagnostica genetica. Sorbona

  2. Laposata M. Medicina di Laboratorio. Piccin

  3. Antonozzi I. Medicina di Laboratorio. Piccin

  4. Federici G. Medicina di Laboratorio. Mc Graw-Hill Companies

  5. Balestrieri et al. Diagnostica Molecolare nella Medicina di Laboratorio. Piccin

• Clinical Biochemistry
  

  Medicina di Laboratorio, G. Federici (Autore), – Mc GrawHill Medicina di laboratorio.

  Medicina di Laboratorio. La diagnosi di malattia nel laboratorio clinico, M. Laposata (Autore) - Piccin

  Medicina di Laboratorio. Logica e patologia clinica, I. Antonozzi, E. Gulletta (Autori) - Piccin

  Medicina dell'Aging e dell'Antiaging. V. Calabrese et al. (Autori) - Edra

  Nutrigenomica e Epigenetica. V. Calabrese et al. (Autori) - Edra

• Clinical microbiology
  

  1 ROBERTO CEVENINI : MICROBIOLOGIA CLINICA

  2 P. R. MURRAY : MICROBIOLOGIA MEDICA

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