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BIOLOGIA CELLULARE E PATOLOGIA

Teaching Staff

DANILO GIUSEPPE VITALE - Module Cell Biology - BIO/06 - 3 CFU
KATIA DOMENICA MANGANO - Module PATOLOGIA - MED/04 - 6 CFU

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Learning Objectives

• Cell Biology
  

  The Course purpose is the in-depth knowledge in the applicative field of Cell Biology.
  More specifically, the course aims to examine in depth the molecular composition and the functional features of some molecules involved in cell-cell/cell-matrix adhesion systems, paying particular attention to the cytoskeletal components and their pathological alterations.

  The course will analyze also the biological/clinical applications of the stem cells (SC) and the in vitro/in vivo methods of study of these cells: the main mechanisms that regulate the differentiation process and self-renewal of SC will be analyzed, including the role of growth factors, cytokines and transcription factors.

  The course also aims to provide the students with the methodological skills related to the identification of some studied cell molecules and in vivo/in vitro techniques for investigations on stem cells .

• PATOLOGIA
  

  At the end of the course the student shoul be able to understand the different causes and the pathogenetic mechanisms of human diseases, as well as the etiopathogenesis of the fundamental alterations of the structures, functions and control mechanisms. They also have to know the fundamental biological mechanisms of defense and their alterations. The course will develop from the molecular level to the higher levels of organization (cellular, tissue, organ, apparatus, organism)

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Detailed Course Content

• Cell Biology
  

  1) Cytoskeleton and cell motility

  Organization and functional features of the structural elements of the cytoskeleton.

  Interactions between cytoskeletal components.

  Analysis methods for the study of the cytoskeletal functions.

  Pathological alterations of cytoskeletal components.

   

  2) Interactions between cell surface and environment

  Extracellular matrix.

  Cell-cell/cell-matrix interactions.

  Membrane receptors and adhesion molecules: immunoglobulins, cadherins, integrins, lectins, selectins.

  Necrosis and Apoptosis: cell and molecular aspects.

  Methods used for the identification of molecules involved in cell-recognition and cell-adhesion.

  Pathological alterations of the cell adhesion mechanisms.

   

  3) Stem cells and nuclear reprogramming

  Stem cells: introduction and background.

  Differentiation potential of stem cells.

  Stem cells: embryonic, embryonic germ, fetal, amniotic, adult.

  Investigative tecniques, in vivo and in vitro, of stem cells.

  Techniques of Nuclear reprogramming: nuclear transfer, cell fusion, transduction of transcription factors.

  Role of epigenetic events in nuclear reprogramming.

  Technological aspects and applications in the clinical setting.

   

  Some scientific studies regarding the abovementioned topics will be examined and discussed critically during the Course.

  Laboratorial activities will be also carried out for learning some standard techniques used in the investigations related to some of the abovementioned topics (therefore, certificate showing the frequency of Safety Course is required and it is compulsory to wear a lab coat) .

• PATOLOGIA
  

  Frontal lessons
  • Introduction of general pathology
  • Alterations of base-acid balance
  • Genetic diseases
  • Environmental pathology
  • Cellular pathology: cellular adaptations (hyperplasia, hypertrophy, atrophy, metaplasia, dysplasia), apoptosis, necrosis
  • Regeneration, repair and healing
  • Inflammation: acute, chronic, mediators of inflammation
  • Fever and temperature changes
  • Immune system: innate immunity, acquired (mediated and humoral cell)
  • Elusion of immune defence
  • Primary and acquired immunodeficiencies (AIDS)
  • Hypersensitivity: I, II, III and IV type
  • Immune tolerance
  • Autoimmunity: pathogenic mechanisms and some autoimmune diseases (multiple sclerosis, rheumatoid arthritis, type I diabetes)
  • tumors
  • Atherosclerosis
  • Amyloidosis

  
  Exercises
  • Safety regulations in the pathology and immunology laboratory.
  • Correct use of reagent and equipment supplied to the laboratory
  • Cell cultures of primary and tumor cells
  • Immunological techniques
  • Basic techniques in molecular biology

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Textbook Information

• Cell Biology
  

  1) Alberts B. et al. - Biologia molecolare della cellula - Zanichelli

  2) Bacci S. et al. (Cur.: Colombo R., Olmo E.) - Biologia. Cellula e Tessuti - Edi-Ermes

  3) Cooper G.M., Hausman R.E. - La cellula. Un approccio molecolare - PICCIN

  4) Lewin B. et al. - Le cellule - Zanichelli

  5) Materials (ppt) will be provided during the course

• PATOLOGIA
  

  Elementi di patologia generale per corsi di laurea in professioni sanitarie, Giovanni M. Pontieri – Piccin

  Patologia generale di Maurizio Parola

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