A. CITOLOGY
- Biological characteristics of living matter.
- Chemical characteristics of living matter: water, mineral salts, carbohydrates, lipids, proteins, nucleic acids.
- Levels of organization of living matter.
- Eukariotic animal cell.
- Plasma membrane and glycocalyx. Differentiations of the plasma membrane: microvilli, cilia and flagella, cell junction, basal lamina.
- Cytoplasm: hyaloplasm; ribosomes; rough endoplasmic (RER) and smooth (REL) reticulum; Golgi apparatus; lysosomes; peroxisomes; mitochondria; cytoplasmic inclusions; pigments; cytoskeleton and its constituents; centrioles and centrosomes. Endocytosis and exocytosis.
- Nucleus and its constituents: nuclear envelope; nucleoplasm and nucleoskeleton; organization and functions of chromatin; chromosomes and chromosomes set; nucleolus.
- Cell cycle and its phases. Interkinesis: G0, G1, S, G2 phases (generality about: transcription, protein synthesis, duplication of DNA).
- Cell division: mitosis and meiosis. Gametogenesis (generality).
B. INSTRUMENTS AND METHODS OF INVESTIGATION
- Direct observation of living cells and tissues; vital stains and supravitally.
- Procedures for preparation of permanent slides.
- Main histological staining.
- General principles of cytochemistry and histochemistry.
- Optical and electronic microscopes: operating principles and their use in biology.
C. HYSTOLOGY
The animal tissue: classification and recognition.
- Epithelial tissue: general characteristics and classification.
- Glandular tissue: origin and classification of glands; characteristics of secretory cells and various types of secretion.
- Connective tissues: characteristics of the intercellular substance: ground substance and connective fibers; connective cells.
- Connective tissues proper.
- Specialized connective tissues: cartilage (hyaline, elastic, fibrous) bone and chordoid tissue. Bone development: intramembranous and endochondral ossification.
- Adipose tissues.
- Blood, plasma, and formed elements. Endothelium and blood vessels. Lymph.
- Muscle tissue: smooth muscle, striated skeletal and cardiac muscle.
- Nervous tissue: neuron or nervous cell, morphology and structure. Types of nerve fibers and their structures. Synapses and nerve endings. Neuroglia.
D. PRELIMINARY KNOWLEDGE
- Elementary knowledge of the organization of living matter, including viruses, prokaryotic, eukaryotic cell.
- General principles of physics with particular reference to the diffusion of gases and optical phenomena.
- Basic knowledge of general, inorganic and organic chemistry, with particular reference to: chemical bonds, ions, acids, bases, salts, true solutions, suspensions and colloidal solutions; main classes of organic molecules.