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BIOLOGIA CELLULARE E PATOLOGIA

Teaching Staff

ANNA MARIA PAPPALARDO - Module Cell biology - BIO/06 - 3 CFU
SAVERIO CANDIDO - Module General Pathology - MED/04 - 7 CFU

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Learning Objectives

• Cell biology
  

  The course aims to deepen the knowledge in the field of Cell Biology applications. More specifically, the course aims to analyze the relative knowledge
  to the molecular composition and functional properties of some of the molecules involved in cell-cell and cell-matrix adhesion systems, paying particular attention to the
  cytoskeletal component and possible pathologies related to possible alterations of these molecules. A part of the course will be dedicated to the knowledge of the basic procedures and the main study methods necessary to cultivate eukaryotic cells with particular attention to stem cells (CS) and their role in the clinical practice of the future will also be discussed.

• General Pathology
  

  The aim of general pathology course is to provide the basis for understanding the pathological mechanism involved in development of human diseases. During the course, the causes of diseases (etiology), the mechanism by which the etiological agents cause pathological condition (pathogenesis) will be discussed. Finally, will be elucidated the molecular and cellular mechanisms that allow the organism to counteract the effects of pathogens and other harmful agents.

Course Structure

• Cell biology
  

  Lectures, exercises and discussion of scientific works on the topics covered.

  Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
  
  

  Verification of learning: learning assessment may also be carried out on line, should the conditions require it.

• General Pathology
  

  Frontal lesson.

  Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

  The learning assessment consists of an oral test on the contents indicated in the program.

  Learning assessment may also be carried out on line, should the conditions require it.

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Detailed Course Content

• Cell biology
  

  1) Cytoskeleton and cell motility. Organization and functional characteristics of the structural elements of the cytoskeleton. Interactions between cytoskeletal components. Analysis methods for the study of cytoskeletal functions. Pathologies related to alterations of cytoskeletal components.

  2) Interactions between cells and the surrounding environment. Extracellular matrix. Cell-cell and cell-matrix interactions. Membrane receptors and adhesion molecules: immunoglobulins, cadherins, integrins, lectins, selectins. Necrosis and Apoptosis: cellular and molecular aspects. Pathologies related to alterations of cell adhesion mechanisms.

  3) ROS and oxidative stress. Cellular antioxidant defense systems. Nrf2 and the response to oxidative stress.

  4) Introduction to cell cultures. Continuous or primary cell lines, in adhesion and in suspension. Maintenance in culture and expansion of cell lines in adhesion and in suspension. Mobile phone account.

  5) Stem cells and nuclear reprogramming. Stem cells: generalities and historical outlines. Stem cell differentiation potential. Stem cells: embryonic, fetal, amniotic, adult. In vivo and in vitro techniques for studying stem cells. Technological aspects and applications in the clinical field.

• General Pathology
  

  Frontal lessons

  • Introduction of general pathology

  • Alterations of base-acid balance

  • Genetic diseases

  • Environmental pathology

  • Cellular pathology: cellular adaptations (hyperplasia, hypertrophy, atrophy, metaplasia, dysplasia), apoptosis, necrosis

  • Regressive processes of cell

  • Pathology of extracellular matrix

  • Regeneration, repair and healing

  • Inflammation: acute, chronic, mediators of inflammation

  • Fever and temperature changes

  • Immune system: innate immunity, acquired (mediated and humoral cell)

  • Elusion of immune defence

  • Primary and acquired immunodeficiencies (AIDS)

  • Hypersensitivity: I, II, III and IV type

  • Immune tolerance

  • Autoimmunity: pathogenic mechanisms and some autoimmune diseases (multiple sclerosis, rheumatoid arthritis, type I diabetes)

  • Tumors
  

  
  Exercises
  • Safety regulations in the pathology and immunology laboratory.
  • Correct use of reagent and equipment supplied to the laboratory
  • Cell cultures of primary and tumor cells
  • Immunological techniques
  • Basic techniques in molecular biology

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Textbook Information

• Cell biology
  

  1) Alberts B. et al. - Biologia molecolare della cellula - Zanichelli
  2) Karp G. - Biologia cellulare e molecolare - EdiSES
  3) Hardin J, Bertoni G.P. - Becker. Il mondo della cellula - Pearson
  4)Teaching materials (pdf files) will be provided during the course.

• General Pathology
  

  Elementi di patologia generale per corsi di laurea in professioni sanitarie, Giovanni M. Pontieri – Piccin

  Patologia generale di Maurizio Parola

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Open in PDF format Versione in italiano