CHIMICA E BIOTECNOLOGIE DELLE FERMENTAZIONI

This course aims to provide the theoretical bases of the strategies employed to set-up, manage and improve microbial biotechnological processes, for the industrial production of compounds.

Focus is on bioreactor technology, scale-up strategies, understanding the importance of metabolic pathways on process development and on metabolic engineering strategies utilized to engineer microorganisms in the context of biotechnological applications.

Students will develop the capabilities for setting-up and manage an industrial fermentation process using microbial cells. More in detail they will acquire specific tools (bioreactor set-up and fermentation control, strain improvement, metabolic flux analysis and engineering, statistical analyses) in order to meet the demand of high productive and efficient industrial bioprocesses.

Teaching methods: Frontal lessons (42 hrs)

Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
For a successful learning, basic knowledge of Chemistry, Biology and Biochemistry is required.

Slides of the lessons will be available for students on the "Studium" platform.

Assessment: Oral exam.

Learning assessment may also be carried out on line, should the conditions require it.

Information for students with disabilities and / or SLD: To guarantee equal opportunities and in compliance with the laws in force, interested students can ask for a personal interview in order to plan any compensatory and / or dispensatory measures, based on the teaching objectives and specifications needs. It is also possible to contact the CInAP contact person (Center for Active and Participatory Integration - Services for Disabilities and / or SLD) of the Department of Biomedical and Biotechonological Sciences.

Prokaryotic and Eukaryotic Cells in Biotech Production. The fermenters (bioreactors).

Fermentation technologies: factors affecting microbial growth, batch fermentation, continuous fermentation, fed-batch fermentation, industrial media.

Products of primary metabolism and biomass (ethanol, organic acids, aminoacids, enzymes and proteins)

Production of secondary metabolites (antibiotics, vitamins)

Recombinant Protein Production (enzymes, monoclonal antibody, hormones)

Food productions.

Produzioni alimentari.

Civil and industrial wastewater treatment.

• Biotecnologie e chimica delle fermentazioni di Alberto Tagliaferri, Celeste Grande - Zanichelli - 2002

• Biotecnologie microbiche. di Stefano Donadio e G. Marino - CEA - 2008

• Chimica e biotecnologia delle fermentazioni industriali di Michele M. Bianchi - Nuova Cultura - 2009

• Fermentation Microbiology and Biotechnology, Fourth Edition - E. M. T. El-Mansi, Jens Nielsen, David Mousdale, Ross P. Carlson - CRC Press, 2018 (testo di consultazione)