Bachelor's Degree in Biomedical Laboratory techniques
Academic Year 2021/2022 - 2° Year
METODICHE DIAGNOSTICHE DI MICROBIOLOGIA CLINICA E PARASSITOLOGIA
Teaching Staff
MARIO SALMERI - Module TECNICHE IN DIAGNOSTICA MICROBIOLOGICA - MED/07 - 3 CFUILDEBRANDO MARIA CONCETT PATAMIA - Module Parasitic anthropozoonosis - VET/06 - 3 CFU
Learning Objectives
- TECNICHE IN DIAGNOSTICA MICROBIOLOGICA
TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY
Knowledge of applications foundation for microbiological sciences in the various sectors of health interest
Knowledge of the main microbial identification techniques and acquisition of the skills to apply the same in health care
Knowledge of the "Guidelines for sterilization on the activity as a collective protection against biological agents for the operator in health facilities (Legislative Decree no. 81/2008 and subsequent amendments)"
- Parasitic anthropozoonosis
Knowledge of the main human parasitic anthropozoonoses. Epidemiology, life cycles of parasites, basic knowledge on the pathogenesis of the most relevant parasitic diseases and their main clinical manifestations. Diagnostic methods in intestinal and blood parasitology: Traditional Direct, Immunochromatographic, Biomolecular. Serological Diagnostics in Parasitology.
Course Structure
- TECNICHE IN DIAGNOSTICA MICROBIOLOGICA
TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY
Lectures
Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
Learning assessment may also be carried out on line, should the conditions require it.”
- Parasitic anthropozoonosis
Frontal lessons
Detailed Course Content
- TECNICHE IN DIAGNOSTICA MICROBIOLOGICA
TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY Principles of Microbiology
Microbes and metazoa
Principles of microbial nutrition and metabolism
Methods of microbial taxonomy and nomenclatureMetodi biochimiciMolecular methods
Other methods (not molecular) New approaches to taxonomy Omic SciencesDiagnostics of bacterial diseases
The methods of sampling
Transport media for bacteriologyMicroscopic examination Staining techniques
Direct diagnostics Cultivation methods
Culture media
Aerobic and anaerobic growth
Microorganisms that require special nutrients and/or cells for growth Biochemical tests for identificationManual methods
Automated methods Blood Culture
Culture independent and molecular techniques Immunofluorescence
Enzyme immuno assay Other methods Molecular methods
Hybridization Probes
Amplification techniques
Typing systems
Other culture independent techniquesIndirect diagnostic: Serological methods
Immunofluorescence Enzyme immuno assay Other methods
Susceptibility testing and bioassay
In vitro antibiotic susceptibility testingDilution methods Diffusion methods Automated methods
Bioassays
Pyrogenes and bacterial endotoxins Pyrogenes assay
Assay for bacterial endotoxins (LAL test) Biological Assay of antibiotics
Assay of interferons
Assessment of the activity of disinfectantsDiagnostic of viral diseases
The methods of sampling Cultivation method
The components required for cell culture media. Primary and Secondary cell cultures Semi-continuous diploid cells (Cell strains) Continuous cell lines
Culture independent and molecular techniques Enzyme Immuno assay
Immunofluorescence Molecular methods
Hybridization
Probes
Amplification techniques Typing systemsOther culture independent techniques Serological methods
Immunofluorescence Enzyme immuno assay Other methods
Antiviral chemotherapy
The assay of substances with potential antiviral activityDiagnostic of fungal diseases
The methods of sampling Microscopy and culture
Staining techniques
Mycological culture media
Biochimical test for fungal identificationManual
Automated
Culture independent and molecular techniquesImmunofluorescence Enzyme immuno assay
Other methods
Molecular methods Hybridization
Probes
Amplification techniques
Typing systems
Other culture independent techniquesSerological methods Immunoflurescence
Enzyme iImmuno assay
Other technique Antimycotic susceptibility besting
Dilution methods Yeast Molds
Other technique
The microbiological quality control and hygiene environment work [Notes on guidelines for the activity of sterilization as a collective protection against biological agents for the operator in health facilities (D.Lgs. 81/2008 e s.m.i.)]
The sterilization process and its enterprise management People involved
Structural and technological requirements.Large / medium-sized hospitals
Small hospitals Operating protocols
Harvesting
Decontamination
Washing (manual, ultrasaund, automated)
Personal protective equipment [gloves, protective clothing, respiratory protection devices, for face protection (face shield or equivalent)]Rinsing, drying, inspection and maintenance
Medical paper, envelopes and rolls in paper-laminated polymeric film, a polymeric material composition of various types usable in sheets, polyolefin and similar materials suitable for use in rolls or tubular, reusable Material: container
Sterilization (steam sterilization, with ethylene oxide, with other physical methods, gas plasma of hydrogen peroxide, peracetic acid solutions
Controls on environments and the process stepsFood Microbiology - HACCP System
HACCP system
Description of the HACCP systemPreliminary phases of the application system Main features of HACCP system
Principles 1 to 7 Food microbiology
Meaning of microorganisms in food
Unfermented foods
Meat, poultry, eggs, seafood, vegetables and fruit Fermented foods
Fermented Vegetables, bread, cheese, fermented milk, fermented meat products
- Parasitic anthropozoonosis
General parasitology, basic notions. Helminthology: Nematodes, Cestodes, Trematodes. Morphological description, life cycles, notions of epidemiology. Description of the main helminths responsible for anthropozoonosis, hints of pathogenesis, diagnostic techniques. Protozoology: Morphological description, life cycles, notions of epidemiology. Description of the main Protozoa responsible for anthropozoonosis, hints of pathogenesis, diagnostic techniques.
Textbook Information
- TECNICHE IN DIAGNOSTICA MICROBIOLOGICA
TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY
Koneman's - Color Atlas and textbook of Diagnostic Microbiology 7ed. Wolters Kluver - International Edition ISBN-13: 978-1-4511-8935-3
Other informations and papers will be available in Studium
- Parasitic anthropozoonosis
De Carneri Parassitologia medica e diagnostica parassitologica.
Open in PDF format Versione in italiano