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MEDICINA DI LABORATORIO E DIAGNOSTICA INTEGRATA - channel 2

Teaching Staff

ERNESTINA MARIANNA DE FRANCESCO - Module Clinical Pathology - MED/05 - 2 CFU
VITTORIO CALABRESE - Module Clinical Biochemistry - BIO/12 - 2 CFU
GUIDO SCALIA - Module Microbiology and Clinical Microbiology - MED/07 - 2 CFU

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Learning Objectives

• Clinical Pathology
  

  The course is aimed at building knowledge and developing competences in the field of Clinical Pathology.

  The students will learn to evaluate and interpret the laboratory tests used in the main hematological diseases, in the alterations of the coagulation system, in the alterations of liver, in certain endocrinopathies, in autoimmune diseases, in periodontal diseases and neoplastic diseases.

• Clinical Biochemistry
  

  The course is designed to provide students with a good foundation of knowledge in all aspects of Clinical Biochemistry. Introducing the fundamentals of instrumentation and methodology in the clinical chemistry and biochemistry laboratory medicine, lectures will cover urinalysis testing procedures and associated disease entities, as well as analysis of other body fluids. Clinical testing using automated and manual methods, measurement of pancreatic function and intestinal absorption, renal and liver function, enzymes, electrolytes, blood gases, lipids, toxicology, urinalysis, endocrinology, neurological, dysmetabolic, inflammatory and cardiovascular diseases.

• Microbiology and Clinical Microbiology
  

  Knowledge of prevention and prophylaxis criteria of infections and possible therapeutic opportunities; pathology and pathogenesis of the main infectious agents involved in human pathology; paths of follow-up, diagnosis, and therapy; apply the knowledge of microbiology to human disease by systems or organs.

Course Structure

• Clinical Pathology
  

  The lessons will be held in classrooms. PDF presentations, which will be available for the students on the platform Studium, will be used as a presentation tool. Despite representing a facilitating tool during the learning process, the PDF presentations do not represent a replacement for textbooks.Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

  Oral exam will be used for learning assessment. Learning assessment may also be carried out on line, should the conditions require it.

• Clinical Biochemistry
  

  Frontal teaching, elaboration, Individual study

• Microbiology and Clinical Microbiology
  

  Lectures in the classroom with the teacher.

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Detailed Course Content

• Clinical Pathology
  

  Laboratory tests: definition and types.

  Collection and storage of biological samples.

  Pre-analytical, analytical and biological variability.

  Blood tests.

  Plasma Proteins and Electrophoresis.

  Hemostasis and Coagulation.

  Blood groups and Compatibility tests.

  Diabetes: diagnosis and follow-up.

  The liver: diagnosis of viral hepatitis.

  Diagnostics in autoimmune diseases.

  Diagnostics in periodontal diseases.

  Tumor markers.

• Clinical Biochemistry
  

  1) The use of laboratory data in clinical practice
  2) The method of acquisition of laboratory data
  3) The interpretation of biochemical data
  4) Analysis of Urine
  5) Biochemistry of nutrition
  6) Assessment of liver function and diagnosis of jaundice
  7) Acute and chronic diseases of the liver
  8) Liver function and Hepatitis tests
  9) Diabetes mellitus
  10) Hypoglycemia
  11) Lipid metabolism, dyslipidemia and atherosclerosis, sphingolipidoses.
  12) Free radicals in the pathology
  13) Metabolism of ethanol and the pathology of alcoholism
  14) Complete blood count test
  15) Disorders of porphyrin and heme synthesis, hemoglobinopathies
  16) Plasma proteins and electrophoresis
  17) Blood groups and compatibility tests
  18) Hemostasis tests
  19) Kidney function tests and urine test
  20) Iperammoniemie;
  21) Metabolism of amino acids (Phenylketonuria, Hyperhomocysteinemia);
  22) Disorders salt and water balance and acid-base balance;
  23) Disorders of purine metabolism (hyperuricemia, gout)
  24) Investigations on the cerebrospinal fluid
  25) Biochemical aspects of neurological diseases
  26) Tumor markers
  27) Metabolic effects of tumors
  28) Cellular aspects of clinical biochemistry
  29) Molecular Clinical Biochemistry
  30) Clinical biochemistry in the diagnosis of acute pain in the chest and abdomen
  31) Biochemistry and molecular clinical biology of organ transplantation
  32) Notes on antiaging medicine.

• Microbiology and Clinical Microbiology
  

  GENERAL PRINCIPLES of Clinical Microbiology: microbial ecology; pathogenicity and virulence; infection and disease; epidemiology of infectious diseases; nosocomial infections.

  METHODS in Clinical Microbiology: Clinical Question and requests for investigation; direct and indirect diagnosis; sampling; elements of microbiological techniques; interpretation of the response.

  CLINICAL MICROBIOLOGY: Aetiological agent, symptoms, clinical sample, prevention, prophylaxis, therapy, and diagnosis of infections of:

  upper respiratory tract; lower respiratory tract; cardiovascular apparatus; gastrointestinal apparatus; hematopoietic apparatus; central nervous system; sexually transmitted diseases; urogenital apparatus; eye; ear, nose and throat; skin and mucous membranes; wound infections; bones and articulations; immunocompromised host infection; TORCH; diagnosis prevention and follow-up of HPV infections.

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Textbook Information

• Clinical Pathology
  

  1. Federici G. Medicina di Laboratorio. Mc Graw-Hill Companies

  2. Laposata M. Medicina di Laboratorio. Piccin

  3. Ciaccio M., Lippi G. Biochimica clinica e Medicina di Laboratorio. EdiSES

  4. Antonozzi I. Medicina di Laboratorio. Piccin

  5. Balestrieri et al. Diagnostica Moleculare nella Medicina di Laboratorio. Piccin

• Clinical Biochemistry
  

  Medicina di Laboratorio, G. Federici (Autore), – Mc GrawHill Medicina di laboratorio.

  Medicina di Laboratorio. La diagnosi di malattia nel laboratorio clinico, M. Laposata (Autore) - Piccin

  Medicina di Laboratorio. Logica e patologia clinica, I. Antonozzi, E. Gulletta (Autori) - Piccin

  Medicina dell'Aging e dell'Antiaging. V. Calabrese et al. (Autori) - Edra

  Nutrigenomica e Epigenetica. V. Calabrese et al. (Autori) - Edra

• Microbiology and Clinical Microbiology
  
  1. Antonelli, Clementi, Pozzi, Rossolini : Principi di Microbiologia medica – Ed. Ambrosiana
  2. La Placa : Principi di Microbiologia Medica – Ed. Esculapio
  3. Jawetz, Melnick, Adelberg’s: Microbiologia medica – Ed. Piccin
  4. Sherris: Microbiologia Clinica, Ed. EMSI
  5. Murray et al.: Microbiologia Clinica - Elsevier

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