ANALYTICAL CHEMISTRY II AND LABORATORY M - Z

CHIM/01 - 7 CFU - 2° Semester

Teaching Staff

ROBERTA D'AGATA


Learning Objectives

The aim of the course is to provide basic principles of instrumental analysis. After finishing the course, students should be able to assess potential, advantages and limitations of the discussed methodologies with emphasis on spectroscopy and electrochemical techniques.


Course Structure

Lectures on theory are complemented with practical laboratory experiences in small groups working in Lab. During the lectures it will provide all information needed to allow for the understanding and the implementation of laboratory operations along with the critical assessment and interpretation of the analytical results of testing methods and the presentation of procedures and results in concise written reports.



Detailed Course Content

1. Spectroscopy

Electromagnetic radiation: photoelectric effect, diffraction, mathematical representation, electromegnetic spectrum, refraction, refraction index, Snell law, total internal reflection. Radiation-matter interaction: replection, transmission/adsorption, diffusion( Rayleigh, Mie, Raman). Polarization. Adsorption and emission of radiation. Atomic adsorption.

Molecular adsorption: vibration and rotation. Fluorescence and phosphorescence. Jablonski diagram.

2. UV-vis spectroscopy

Trasmittance, adsorbance, Beer’s law: limit, chemical deviations, instrumental deviations. Photometers and spectrophotometers. Spetrophotometers: single beam, double beams. Sources. Filters and monochromators. Prisms. Transducers.

 

3. Infrared spectroscopy

Armonic and anarmonic oscillators. Vibrational modes, Fourier transform. Michelson interferometer. Sources. Infrared spectra. Sample requirements. Diffuse reflectance. ATR. Ifrared microscopy.

 

Raman spectroscopy

Raman effect. Polarizability. Comparison with IR spectroscopy. Sources. Raman microscopy.

Electroanalytical methods. Potentiometry

X-ray Fluorescence.

Laser

Statistics.

Diodes. LED, Operational amplifiers.



Textbook Information

-Skoog, Holler, Nieman , "PRINCIPLES OF INSTRUMENTAL ANALYSIS" , Brooks Cole.




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