MEDICINA DI LABORATORIO E DIAGNOSTICA INTEGRATA - channel 2

6 CFU - 1° Semester

Teaching Staff

LIVIA MANZELLA - Module Clinical Pathology - MED/05 - 2 CFU
VITTORIO CALABRESE - Module Clinical Biochemistry - BIO/12 - 2 CFU
GUIDO SCALIA - Module Microbiology and Clinical Microbiology - MED/07 - 2 CFU

Learning Objectives

Clinical Pathology
The course develops skills in the Clinical Pathology aiming to teach the student to apply, evaluate and interpret the laboratory tests used to study of the main diseases of the blood, alterations of the coagulative-fibrinolytic system, changes in liver and kidney function, some endocrinopathies.

1. Knowledge and understanding: at the end of the course, students will acquire the knowlodge of the main laboratory tests to the study the blood diseases, the coagulative-fibrinolytic system, liver and kidney function alterations andsome endocrinopathies.

2. Ability to apply knowledge and understanding: once the knowledge of the main laboratory tests has been acquired, the students will be able to evaluate and interpret the aforementioned tests.

3. Making judgments: through the executions, the students will be able, independently, to apply, evaluate and interpret different laboratory tests.

4. Communication skills: the student will acquire the necessary communication skills within the main laboratory tests.

5. Learning skills: the aim of the course is to provide the student with the basic knowledge to autonomously interpret the main laboratory tests.
Clinical Biochemistry
The course is designed to provide students with a good foundation of knowledge in all aspects of Clinical Biochemistry. Introducing the fundamentals of instrumentation and methodology in the clinical chemistry and biochemistry laboratory medicine, lectures will cover urinalysis testing procedures and associated disease entities, as well as analysis of other body fluids. Clinical testing using automated and manual methods, measurement of pancreatic function and intestinal absorption, renal and liver function, enzymes, electrolytes, blood gases, lipids, toxicology, urinalysis, endocrinology, neurological, dysmetabolic, inflammatory and cardiovascular diseases.
Microbiology and Clinical Microbiology
Knowledge of prevention and prophylaxis criteria of infections and possible therapeutic opportunities; pathology and pathogenesis of the main infectious agents involved in human pathology; paths of follow-up, diagnosis, and therapy; apply the knowledge of microbiology to human disease by systems or organs.

Course Structure

Clinical Pathology
Lessons are held in the classroom with the help of PDF presentations made available to students on the Studium portal. The presentations do not replace the reference texts, but serve as educational support for the program carried out.
Clinical Biochemistry
Frontal teaching, elaboration, Individual study
Microbiology and Clinical Microbiology
Lectures in the classroom with the teacher.

Detailed Course Content

Clinical Pathology
Laboratory analysis: definition, typology

Collection and conservation of biological samples

Preanalytical, analytical and biological variability

Complete blood count test

Plasma proteins and electrophoresis

Hemostasis test

Blood groups and compatibility testing

Urine analysis and functional kidney test

The functional liver test

Diabetes: diagnosis and follow up tests

Tumoral Markers

Autoimmune disease tests
Clinical Biochemistry
1) The use of laboratory data in clinical practice
2) The method of acquisition of laboratory data
3) The interpretation of biochemical data
4) Analysis of Urine
5) Biochemistry of nutrition
6) Assessment of liver function and diagnosis of jaundice
7) Acute and chronic diseases of the liver
8) Liver function and Hepatitis tests
9) Diabetes mellitus
10) Hypoglycemia
11) Lipid metabolism, dyslipidemia and atherosclerosis, sphingolipidoses.
12) Free radicals in the pathology
13) Metabolism of ethanol and the pathology of alcoholism
14) Complete blood count test
15) Disorders of porphyrin and heme synthesis, hemoglobinopathies
16) Plasma proteins and electrophoresis
17) Blood groups and compatibility tests
18) Hemostasis tests
19) Kidney function tests and urine test
20) Iperammoniemie;
21) Metabolism of amino acids (Phenylketonuria, Hyperhomocysteinemia);
22) Disorders salt and water balance and acid-base balance;
23) Disorders of purine metabolism (hyperuricemia, gout)
24) Investigations on the cerebrospinal fluid
25) Biochemical aspects of neurological diseases
26) Tumor markers
27) Metabolic effects of tumors
28) Cellular aspects of clinical biochemistry
29) Molecular Clinical Biochemistry
30) Clinical biochemistry in the diagnosis of acute pain in the chest and abdomen
31) Biochemistry and molecular clinical biology of organ transplantation
32) Notes on antiaging medicine.
Microbiology and Clinical Microbiology
GENERAL PRINCIPLES of Clinical Microbiology: microbial ecology; pathogenicity and virulence; infection and disease; epidemiology of infectious diseases; nosocomial infections.

METHODS in Clinical Microbiology: Clinical Question and requests for investigation; direct and indirect diagnosis; sampling; elements of microbiological techniques; interpretation of the response.

CLINICAL MICROBIOLOGY: Aetiological agent, symptoms, clinical sample, prevention, prophylaxis, therapy, and diagnosis of infections of:

upper respiratory tract; lower respiratory tract; cardiovascular apparatus; gastrointestinal apparatus; hematopoietic apparatus; central nervous system; sexually transmitted diseases; urogenital apparatus; eye; ear, nose and throat; skin and mucous membranes; wound infections; bones and articulations; immunocompromised host infection; TORCH; diagnosis prevention and follow-up of HPV infections.

Textbook Information

Clinical Pathology
1. Ciaccio M. e Lippi G. Biochimica Clinica e Medicina di Laboratorio. EdiSES

2. Federici G. Medicina di Laboratorio. Mc Graw-Hill Companies

3. Laposata M. Medicina di Laboratorio. Piccin

4. Antonozzi I. Medicina di Laboratorio. Picci
Clinical Biochemistry
Medicina di Laboratorio, G. Federici (Autore), – Mc GrawHill Medicina di laboratorio.

Medicina di Laboratorio. La diagnosi di malattia nel laboratorio clinico, M. Laposata (Autore) - Piccin

Medicina di Laboratorio. Logica e patologia clinica, I. Antonozzi, E. Gulletta (Autori) - Piccin

Medicina dell'Aging e dell'Antiaging. V. Calabrese et al. (Autori) - Edra

Nutrigenomica e Epigenetica. V. Calabrese et al. (Autori) - Edra
Microbiology and Clinical Microbiology
1. Antonelli, Clementi, Pozzi, Rossolini : Principi di Microbiologia medica – Ed. Ambrosiana
2. La Placa : Principi di Microbiologia Medica – Ed. Esculapio
3. Jawetz, Melnick, Adelberg’s: Microbiologia medica – Ed. Piccin
4. Sherris: Microbiologia Clinica, Ed. EMSI
5. Murray et al.: Microbiologia Clinica - Elsevier

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