With the course of Applied Molecular Microbiology, students will broaden and deepen the basic knowledge in the biomolecular field captured with the first degree. The course explores the molecular aspects of pathogenicity, virulence and antibiotic resistance of the major human pathogens, and their clonal relationship. The new identification technologies and study of microorganisms are also addressed (genomes, metagenomes, proteomes etc) as well as the basics of the interaction host parasite including immunological aspects. From the application point of view, they are taken into consideration the vaccine preparations (both traditional and recombinant) as well as some examples of microbial products produced with advanced technologies. The students then, will possess an integrated understanding of biological phenomena and advanced scientific training in morphological / functional level, chemical / biochemical, cellular / molecular and evolutionary aspects of the microbial world.
Acquire knowledge of the applicative basis of clinical microbiology in the various fields of interest for health;
Acquire knowledge of the major microbial identification techniques and skills to apply the same in health care
Know the principles of quality control in clinical microbiology
Three main parts:
1) Study of microrganisms of bio-medical interest; Taxonomy and identification; Host-pathogen relationship interactions; molecular mechanisms of pathogenicity; Genetic and genomic analysis; phylogenetic reconstruction; What a microbial genome is teaching us; Bacteriocins, RNA and transcriptomic
2) Pharmaceutical compounds, antibodies; Vaccins (traditional and molecular-based preparations)
3) Applied medical microbiology, examples
Lectures (5 UFCs) and Laboratory (1 UFC)
Study of microrganisms involved in bio-medical and industrial applications.
Overview of taxonomy and identification of microrganisms. Host-parasite relationships. Molecular mechanisms of pathogenicity.
Molecular biotechnology of microbial systems. Phylo-genomics: the benefit between phylogeny reconstruction and genomic analyses.
Genetic and genomic application in the study of new molecular targets for therapeutic agents.
Screening for microbial Products: potential bacteriocins,.
sRNA regulators reprogram bacteria Enzymes, monoclonal and polyclonal antibodies, nucleic acids as therapeutic agents.
Study of molecular mechanisms of antibiotic-resistance. Vaccines. Traditional and recombinant technologies in medical microbiology.
1. Essential and differential characteristics of the main bacteria, viruses, fungi, protozoa and other parasites responsible for human infections:
2. The different possibilities of infection and spread of infectious diseases;
3. The infection control and general knowledge on the prevention of infectious diseases;
4. Diagnosis of bacterial, fungal, viral and protozoal diseases;
5. The microbiological quality control, diagnostic principles for food microbiology and environmental microbiology in health care.
6. Diagnostic models applied to infections
7. New and old integrated diagnostic methods and polyphasic approaches
1) Koneman's - Color Atlas and textbook of Diagnostic Microbiology 7ed. Wolters Kluver - International Edition ISBN-13: 978-1-4511-8935-3
2) Persing et Al. MOLECULAR MICROBIOLOGY Diagnostic Principles and Practice 2 nd Ed. ASM Press. ISBN 978-1-55581-497-7