The course will provide the knowledge of the morphological and functional characteristics of the human organs to allow the microscopic analysis of the bioptic fragments. In addition, students will acquire the ability to identify specific molecular markers for each organ through immunohistochemical and immunofluorescent analysis.
The Histology module purpose is to provide students with appropriate skills on the processes of histogenesis and human embryology, with a detailed description of human prenatal development in the various stages of development, from the zygote (fertilization) to the embryo (embryogenesis) up to IV week of development and on the fundamental aspects of the tissues of the human body.
3 CFU (21h) of classroom lessons and 3CFU (36h) of laboratory practical exercises
Lectures and exercises.
Lectures program: microscopic structure of the organs. Molecular markers that identify the cells of the organs. Structural investigation techniques through immunohistochemical analysis and in situ hybridization. General principles of immunofluorescence. Antibodies and main characteristics of conjugated antibodies. Strategies for double marking and co-location.
Laboratory exercises: immunohistochemistry and in situ hybridization on organ biopsy samples. Microscope recognition of organ and tissue fragments
Histogenesis mechanisms - First stages of embryonic development - Formation of 3 sheets
germ cells and their differentiation - Stem cells
- Tissues: definition and classification
- EPITHELIAL TISSUE: a) Coating epithelia, b) Glandular epithelia
- TROFOCONNECTIVAL AND SUPPORT Tissues: a) Connective tissue properly so-called b)
Cartilaginous tissue c) Adipose tissue d) Bone tissue
- BLOOD
- MUSCULAR TISSUE: a) Smooth muscle tissue b) Skeletal muscle tissue c) Fabric
cardiac muscle
- NERVOUS Tissue
Conconi Rumio -Principi di Anatomia microscopica con cenni di Istologia e Anatomia Macroscopica- ed EdiSES
EMBRIOLOGIA:
1. Moore, Persaud, Torchia - Lo sviluppo prenatale dell’uomo, 9° ed.- edra
ISTOLOGIA:
2. V. Monesi - ISTOLOGIA 6° ediz.- Piccin
3. Adamo, Comoglio, Molinaro, Siracusa, Stefanini, Ziparo - ISTOLOGIA per i corsi di laurea in
professioni sanitarie – Piccin