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MEDICINA DI LABORATORIO E DIAGNOSTICA INTEGRATA - channel 4

Teaching Staff

LUCIA MALAGUARNERA - Module Clinical Pathology - MED/05 - 2 CFU
VINCENZA BARRESI - Module Laboratory Medicine (Clinical Biochemistry and Clinical Molecular Biology (2 CFU) III year, line D - BIO/12 - 2 CFU
MARIA ANTONIETTA TOSCANO - Module Clinical Microbiology - MED/07 - 2 CFU

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Learning Objectives

• Laboratory Medicine (Clinical Biochemistry and Clinical Molecular Biology (2 CFU) III year, line D
  

  Biochemical and molecular alterations, biological samples and molecular tests in Clinical Biochemistry Laboratory

• Clinical Microbiology
  

  Learning of principal scientific fundaments in a modern Laboratory of Clinical Microbiology useful to prepare Students for future knowledge of all methods to reach an exact clinical diagnosis.

  Knowledge of microbiological diagnosis for bacteria, fungi, parasites and virus

  Knowledge of the tests used in microbiological diagnosis

  Knowledge of biological samples suitable for microbiological diagnosis

  Modalities for collection of clinical samples, transport and storage of pathological matherials used for diagnostic purposes

  Interpretation of the results of microbiological tests

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Detailed Course Content

• Laboratory Medicine (Clinical Biochemistry and Clinical Molecular Biology (2 CFU) III year, line D
  

  Definition and role of laboratory medicine, laboratory investigation

  Biological samples: types, sampling, processing and storage. Extraction and assay of nucleic acids.

  The biological report: preanalytical variability, analytical and post-analytical. biological variability. Quality control.

  Diagnostic characteristics of a laboratory test: diagnostic sensitivity, diagnostic specificity, positive predictive value and negative predictive test.

  Liver, metabolism, markers of liver injury, detoxification reactions, alcoholism.

  Heart, markers of cardiac damage, Investigations.

  Cardiovascular risk: atherosclerosis and lipid metabolism, laboratory investigations. Kidney: Parameters for assessment of renal function and urine analysis

  Complete Blood Count (CBC): Results and Interpretation

  • Major disorders of the red blood cell: Anemia, Thalassemia and Hemoglobinopathies, hemolytic anemias, Criteria for assessing the severity of anemia
  • Major disorders of white blood cell: Leukocytosis, Leukopenia, acute and chronic leukemias
  • The genetic diagnosis: Genetic disorders, methods for the analysis of mutations known and not known.
  • Diseases caused by genetic mutations static and dynamic, monogenic and polygenic diseases, mitochondrial inheritance. Prenatal diagnosis Molecular diagnostics in hematology oncology
  • Molecular biology techniques: polymerase chain reaction (PCR), restriction and modification enzymes, immuno assays, sequencing.
• Clinical Microbiology
  

  The micro-organisms which cause infection and infectious diseases: concept of pathogenicity and virulence

  Human microbial flora

  Infectious diseases and host-related infections

  Trasmission of infectious diseases abnd modality of transmission

  Host predisponing condition for infections

  Elements of Microbiological Diagnosis: direct and indirect tests

  Microbial isolation in Laboratory practice.

  Choosing of the most suitable samples for diagnosis

  Principles, aims and interpretation of the Antibiogramma

  Elements of virological diagnostic methods: direct and indirect tests.

  Viral isolation, selection of most suitable samples and methods to preserve and keep them

  Eye infections and diagnostic methods

  Infections of the CNS and diagnostic methods

  Upper respiratory tract infections and diagnostic methods

  Lower respiratory tract infections and diagnostic methods

  Cardiovascular infection, sepsis, bacteremia, and diagnostic methods

  Gastrointestinal tract infections and toxic food infections: diagnostic methods

  Liver infections and diagnostic methods

  Urinary tract infections and diagnostic methods

  Sexually transmitted infections and diagnostic methods

  Female gynaecological infections and diagnostic methods

  Infections in pregnancy: diagnosis of TORCH agents

  Sexually transmitted infections and laboratory diagnosis methods

  Skin, soft tissue infections and laboratory diagnosis methods

  Microbiological control of hospital infections

  Immunocompromised host infections

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Textbook Information

• Laboratory Medicine (Clinical Biochemistry and Clinical Molecular Biology (2 CFU) III year, line D
  

  Recommended reading

  1. Medicina di Laboratorio e Diagnostica Genetica, Autori: L. Sacchetti, P. Cavalcanti, G. Fortunato, L. Pastore, F. Rossano, D. Salvatore, F. Scopacasa. Casa Editrice: Idelson-Gnocchi.

  1. Medicina di Laboratorio, Autori: M. Zatti, Clara Lechi, G. C. Guidi, F. Manzato, G. Lippi. Casa Editrice: Idelson-Gnocchi.

   

  1. Biochimica Clinica.

  Autori: L. Spandrio. Casa Editrice: Idelson-Gnocchi.

   

  1. Biologia Molecolare

  Autori: Francesco Amaldi, Piero Benedetti, Graziano Pesole, Paolo Plevani.

  CEAedizioni

  1. Additional material supplied by the teacher

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