The specific training objectives of the Laboratory Diagnostic Methodologies Course are to enable students to acquire the basic notions for critical evaluation and interpretation of the results of the main diagnostic and biomolecular techniques used in clinical practice and basic research.
1. First generation sequencing: Sanger and Pyrosequencing method.
2. Microarray of gene expression.
3. New generation sequencing NGS - Illumina technology pt 1/2
4. New generation NGS sequencing - Illumina pt 2/2 and Roche 454 technology
5. Digital Droplet PCR.
6. Role of the Microbiota and its implications in oncological and neuropsychiatric pathologies
7. Consultation of scientific literature and design of primers for PCR.
1) Widmann: Interpretazione clinica degli esami di laboratorio. Ronald A. Sacher e Richard A. McPherson. 2001 Undicesima edizione. Mc Graw Hill libri Italia (Milano)
2) Giorgio Federici: Medicina di Laboratorio. 2008 Terza edizione. Mc Graw Hill libri Italia (Milano)