ANALYTICAL CHEMISTRY III AND LABORATORY

The aim of the course is to provide a first framework for the overall synthesis of analytical chemistry, addressing principally the fundamentals and the use of some of the most important instrumental quantitative techniques of analysis and requiring a deep integration of the previously acquired knowledge in the field of analytical chemistry and placed at the end of the study path to exploit the student's maturation.

Optical methods. Absorption and emission spectra. Absorption and atomic spectroscopy. Atomic spectra. Fundamentals of technique and applications. Instrumentation Diagram. Determination of the content of metals in real matrices. Spectrofluorimetry. Fundamentals of qualitative and quantitative analysis. Conductometry. Voltammetry: characterization of a redox system by cyclic voltammetry. Determination of trace analytes with voltammetric pulsed techniques. Chromatographic methods. Introduction to chromatographic techniques. Van Deemter's equation. Column chromatography and thin layer chromatography. Gas chromatography: determination of alcohols in alcoholic distillates. High Performance Liquid Chromatography. Ion chromatography: determination of the content of cations and anions in drinking water. Mass Spectrometry. Instrumentation Diagram. Ionization Techniques. Separation of charged particles. Magnetic, Quadruple, Ion Trap, Time-flight, and Orbital Trap mass analyzers. Advanced micro-separation techniques CE, ITP, MEKC, LECE.

1. D. A. Skoog, Holler, Crouch, Chimica Analitica Strumentale II edizione, EdiSES, Napoli, 2009.

2. D. C. Harris, Chimica Analitica Quantitativa, III edizione, Zanichelli, 2017.

3. Skoog, West, Holler, Crouch, Fondamenti di Chimica Analitica, III edizione, EdiSES, 2015.

4. Skoog, Holler, Crouch, Principles of instrumental analysis - VII ed. Thomson Brooks/Cole, 2007.

5. D. C. Harris, Quantitative Chemical Analysis, 9th ed. W.H. Freeman and Company, New York, US, 2016.