TOSSICOLOGIA E MICROSCOPIA FORENSE

6 CFU - 1° Semester

Teaching Staff

NUNZIATA GIUSEPPA ELISABETTA BARBERA - Module Forensic Toxicology - MED/43 - 3 CFU
VELIA MARIA D'AGATA - Module INDAGINI MICROSCOPICHE - BIO/16 - 3 CFU

Learning Objectives

Forensic Toxicology
The teaching of Forensic Toxicology provides the basic knowledge for the understanding of the mechanisms underlying the poisonings and acute poisoning induced by drugs, illegal drugs, volatile organic compounds (for example ethyl alcohol) and, in general, by xenobiotics. More specifically are studied the analytical techniques to identify and quantify the toxic substances in biological matrices.
INDAGINI MICROSCOPICHE
The course will provide students the knowledge regarding the morphological aspects of the human body. It will allow to acquire the ability to recognize tissues under a microscope from bioptic material. In addition, through practical laboratory exercitations, it will give students the ability to identify molecular markers of pathogenicity or useful for forensic investigations

Detailed Course Content

Forensic Toxicology
Frontal lessons:

- concept of poison

- diagnosis of poisoning

- brief description of the pharmacokinetics (absorption, distribution, metabolism and excretion of drugs)

- methodology of chemical-toxicological survey

- carbon monoxide

- drugs of abuse, ethyl alcohol, opiates and derivatives, cannabis, cocaine, amphetamines, hallucinogens, drugs, volatile substances

- legal aspects of drugs

- ethyl alcohol and driving

- research for drugs in biological and non-organic samples

Laboratory exercises
- Extractive techniques from biological matrices for the detection of: antidepressants and antipsychotics, organic alkaloidal substances, benzodiazepines, opiates, cocaine and metabolites, methadone, cannabinoids, ethyl alcohol, methyl alcohol and volatile substances.
- Specific analytical techniques: screening test, GC-MS, GC-ECD, GC-FID headspace.
INDAGINI MICROSCOPICHE
Microscopic structure of the organs. Molecular markers identifying the cells. Immunohistochemistry and in situ hybridization techniques. General principles of immunofluorescence. Antibodies and conjugated antibodies. Strategies for double labeling and co-location

Textbook Information

Forensic Toxicology
Bertol E, Mari F, Lodi F, Marozzi E. Trattato di tossicologia forense. CEDAM, Padova, 2000.

Bertol E. Analitica tossicologia. Aspetti tecnici interpretativi, giuridici e deontologici, Esculapio, 2011.
INDAGINI MICROSCOPICHE
Papers will be provided to students

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