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METODICHE DIAGNOSTICHE DI MICROBIOLOGIA CLINICA E PARASSITOLOGIA

Teaching Staff

PIO MARIA FURNERI - Module TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY - MED/07 - 3 CFU
ILDEBRANDO MARIA CONCETT PATAMIA - Module ANTROPOZOONOSI PARASSITARIE - VET/06 - 3 CFU

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Learning Objectives

• TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY
  

  Knowledge of applications foundation for microbiological sciences in the various sectors of health interest
  
  Knowledge of the main microbial identification techniques and acquisition of the skills to apply the same in health care
  
  Knowledge of the "Guidelines for sterilization on the activity as a collective protection against biological agents for the operator in health facilities (Legislative Decree no. 81/2008 and subsequent amendments)"

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Detailed Course Content

• TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY
  
  • Principles of Microbiology
    • Microbes and metazoa
    • Principles of microbial nutrition and metabolism
    • Methods of microbial taxonomy and nomenclatureMetodi biochimici
      • Molecular methods
      • Other methods (not molecular)
      • New approaches to taxonomy
      • Omic Sciences

  Diagnostics of bacterial diseases

  • The methods of sampling
    • Transport media for bacteriology
  • Microscopic examination
    • Staining techniques
  • Direct diagnostics
    • Cultivation methods
      • Culture media
      • Aerobic and anaerobic growth
      • Microorganisms that require special nutrients and/or cells for growth
      • Biochemical tests for identification
        • Manual methods
        • Automated methods
      • Blood Culture
    • Culture independent and molecular techniques
      • Immunofluorescence
      • Enzyme immuno assay
      • Other methods
      • Molecular methods
        • Hybridization
          • Probes
        • Amplification techniques
        • Typing systems
        • Other culture independent techniques
  • Indirect diagnostic:
    • Serological methods
    • Immunofluorescence
    • Enzyme immuno assay
    • Other methods
  • Susceptibility testing and bioassay
    • In vitro antibiotic susceptibility testing
      • Dilution methods
      • Diffusion methods
      • Automated methods
    • Bioassays
      • Pyrogenes and bacterial endotoxins
      • Pyrogenes assay
      • Assay for bacterial endotoxins (LAL test)
      • Biological Assay of antibiotics
      • Assay of interferons
      • Assessment of the activity of disinfectants

  Diagnostic of viral diseases

  • The methods of sampling
  • Cultivation method
    • The components required for cell culture media.
      • Primary and Secondary cell cultures
      • Semi-continuous diploid cells (Cell strains)
      • Continuous cell lines
  • Culture independent and molecular techniques
    • Enzyme Immuno assay
    • Immunofluorescence
  • Molecular methods
    • Hybridization
    • Probes
    • Amplification techniques
    • Typing systems
    • Other culture independent techniques
  • Serological methods
    • Immunofluorescence
    • Enzyme immuno assay
    • Other methods
  • Antiviral chemotherapy
    • The assay of substances with potential antiviral activity

  Diagnostic of fungal diseases

  • The methods of sampling
  • Microscopy and culture
    • Staining techniques
    • Mycological culture media
    • Biochimical test for fungal identification
      • Manual
      • Automated
  • Culture independent and molecular techniques
    • Immunofluorescence
    • Enzyme immuno assay
    • Other methods
    • Molecular methods
      • Hybridization
        • Probes
      • Amplification techniques
      • Typing systems
      • Other culture independent techniques
  • Serological methods
    • Immunoflurescence
    • Enzyme iImmuno assay
    • Other technique
  • Antimycotic susceptibility besting
    • Dilution methods
      • Yeast
      • Molds
    • Other technique

  The microbiological quality control and hygiene environment work [Notes on guidelines for the activity of sterilization as a collective protection against biological agents for the operator in health facilities (D.Lgs. 81/2008 e s.m.i.)]

  • The sterilization process and its enterprise management
  • People involved
  • Structural and technological requirements.
    • Large / medium-sized hospitals
    • Small hospitals
  • Operating protocols
    • Harvesting
    • Decontamination
    • Washing (manual, ultrasaund, automated)
    • Personal protective equipment [gloves, protective clothing, respiratory protection devices, for face protection (face shield or equivalent)]
  • Rinsing, drying, inspection and maintenance
  • Medical paper, envelopes and rolls in paper-laminated polymeric film, a polymeric material composition of various types usable in sheets, polyolefin and similar materials suitable for use in rolls or tubular, reusable Material: container
  • Sterilization (steam sterilization, with ethylene oxide, with other physical methods, gas plasma of hydrogen peroxide, peracetic acid solutions
  • Controls on environments and the process steps

  Food Microbiology - HACCP System

  • HACCP system
  • Description of the HACCP system
    • Preliminary phases of the application system
  • Main features of HACCP system
    • Principles 1 to 7
  • Food microbiology
    • Meaning of microorganisms in food
    • Unfermented foods
      • Meat, poultry, eggs, seafood, vegetables and fruit
    • Fermented foods
      • Fermented Vegetables, bread, cheese, fermented milk, fermented meat products

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Textbook Information

• TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY
  

  Koneman's - Color Atlas and textbook of Diagnostic Microbiology 7ed. Wolters Kluver - International Edition ISBN-13: 978-1-4511-8935-3

  Other informations and papers will be available in Studium

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Open in PDF format Versione in italiano