MICROBIOLOGY A - L

MED/07 - 6 CFU - 2° Semester

Teaching Staff

PIO MARIA FURNERI
Email: furneri@unict.it
Office: Dip. Scienze Biomediche e Biotecnologiche BIOMETEC, Sez di Microbiologia, Torre Biologia F. Latteri, Via Santa Sofia, 97. 95123 Catania
Phone: +39 0954781237
Office Hours: tutti i giorni previo appuntamento


Detailed Course Content

  1. The prokaryotic cell
    1. The organization of the bacterial cell
      1. The capsule
      2. The outer membrane and peptidoglycan
      3. The cytoplasmic membrane
      4. The pili
      5. Flagella and bacterial movement
      6. The cytoplasmic organelles
      7. The membrane transport and secretion systems
      8. Bacterial nucleoid
  2. Fungi, Algae, Protozoa and Helminths
    1. Fungi
      1. Characteristics of fungi and their metabolism
      2. The role of fungi in the environment and industry
      3. Important fungi from the health point of view
      4. The main antifungal
    2. Algae
      1. Algae Features
      2. The main phylum of pharmaceutical interest
    3. Protozoa
      1. Characteristics of Protozoa
      2. The important protozoa from the health point of view
      3. The main anti-protozoal drugs
    4. Helminths
      1. Characteristics of Helminths
      2. Flatworms: Flukes and Tapeworms
      3. Nematodes
      4. The main anti-helmintic drug
  3. Viruses and sub- viral structures
    1. The organization of the viral particle
    2. Viral symmetry
    3. Replicative classes
    4. Prions
    5. Viroids and virusoides
    6. The bacteriophage
    7. The antiviral chemotherapy: major agents
  4. The bacterial genetics and cell growth
    1. The notion of genetic information transfer in prokaryotes
    2. Recombination from eukaryotes to prokaryotes
    3. Plasmids
    4. Conjugation,
    5. Transformation
    6. Transduction
    7. Transposable elements
    8. Mutations
    9. The regulation of gene expression: LAC operon
    10. The translational attenuation: the regulation of the TRP synthesis
  5. Bacterial metabolism
    1. Bacterial nutrition and nutritional classification of bacteria
    2. The glycolytic pathways
    3. The gluconeogenesis
    4. Sugars alternative to glucose sugars:
      1. Utilization of polysaccharides (starch, glycogen, cellulose etc)
    5. Metabolism of nitrogen compounds
    6. The tricarboxylic acid cycle and glyoxylate cycle
    7. Bacterial respiration:
      1. oxidative phosphorylation
      2. The anaerobic respiration
    8. Chemiolitotrophy
    9. The fermentative pathways
    10. Features of autotrophic metabolism
    11. Photosynthesis and its diversity
    12. Peptidoglycan synthesis
    13. Protein synthesis
    14. DNA synthesis
  6. Cell growth and differentiation
    1. The cell cycle
    2. The bacterial division
    3. Alternatives to "binary fission"
    4. Vital not cultivable status
    5. The microbial biofilms
    6. The spore and spore-forming bacteria
  7. Host parasite relationships
    1. Bacterial infection
    2. Viral infection
    3. Fungal infection and its classification
    4. Parasitic infection
    5. The host response to infection
    6. Vaccines
  8. Diversity and systematics of the microbial world
    1. Special Bacteriology (taxonomic classification, main characteristics, pathogenicity, infectious diseases, prevention opportunities, diagnostics and chemotherapy )
      1. The main features of: Staphylococcus, Streptococcus, Enterococcus, Neisseria, Branhamella, Mycobacterium, Streptomyces, Nocardia, Actinomyces, Corynebacterium, Lactobacillus, Bartonella, Listeria, Gardnerella, Bacillus, Clostridium, Enterobacteriaceae, Haemophilus, Pasteurella, Vibrio, Legionella, Brucella, Bordetella, Acinetobacter, Pseudomonas, Bacteroides, Campylobacter, Helicobacter, Mycoplasma, Ureaplasma, Chlamydiaceae, Rickettsiaceae, Spirochetaceae.
    2. Special virology (taxonomic classification, main characteristics, pathogenicity, infectious diseases, prevention opportunities, diagnostics and chemotherapy )
      1. The main features of: Poxviridae, Herpesviridae, Adenoviridae, Papillomavirus e Polyomavirus umani,Parvoviridae, Paramyxoviridae, Orthomyxoviridae, Picornaviridae, Arenaviridae, Bunyaviridae, Caliciviridae, Coronaviridae, Filoviridae, Flaviridae, Reoviridae, retrovirus umani, Togaviridae e Rubivirus, virus delle epatiti (HAV, HBV, HCV, HDV,HEV, HGV)
  9. Infection control: sterilization, principles and techniques; disinfection, principles and techniques; The principles of antimicrobial chemotherapySterilization, disinfection and antisepsis
    1. Physical methods
      1. The heat
      2. Radiations
      3. Filtration
    2. Chemical methods
      1. Alkylating agents
      2. Oxidizing agents
    3. Sterility controls
      1. Physical indicators
      2. Chemical indicators
      3. Biological indicators
    4. Sterilization applications in the pharmaceutical field and its limits
      1. Parenteral products
      2. Products non-parenteral
      3. General consideration
    5. Objectives and implementation of the disinfection methods
    6. Targets and mechanisms of action of disinfectants
    7. Types of disinfectants
      1. Phenol derivatives
      2. Biguanide
      3. Surfactant compounds
      4. aldehydes
      5. Halogens
      6. Alcohols
      7. oxidizing agents
    8. Methods for assessing the efficacy of disinfectants
    9. The main antibiotics: mechanism of action and resistance.
  10. The concept of infectious disease: the spread and prevention - Principles of microbiological diagnostic and susceptibility testing
    1. The epidemiology of infectious diseases
      1. General concepts
      2. Epidemiological terminology
      3. Frequency rate
      4. Recognition of an infectious disease within a population
      5. Recognition of an epidemic
      6. The infectious cycle: history of a disease
      7. Carriers and reservoirs
      8. Infectious diseases and emerging and re-emerging pathogensx.
      9. Outbreak control
      10. Clinical and diagnostic methods
      11. Identification of pathogens
      12. Susceptibility to antibiotics (susceptibility testing)
  11. Pharmaceutical production and pharmacopeia
    1. Assessment of pharmaceuticals contamination
      1. Essay of sterility
      2. Evaluation of microbial contamination of non-sterile products
    2. Pyrogens and bacterial endotoxins
      1. Assay for pyrogens
      2. Assays for bacterial endotoxins (LAL test)
    3. Microbiological assay
      1. Biological assay of antibiotics
      2. Interferon dosing
      3. Control the activity of disinfectants
    4. Microbiological characteristics of pharmaceutical products
      1. Obligatorily sterile pharmaceutical preparations
      2. Pharmaceutical preparation does not necessarily sterile
    5. Sterilization of pharmaceutical products
      1. Sterilization methods applicable to pharmaceutical products
      2. Biological sterilization indicators
      3. Use of antimicrobial preservatives in pharmaceutical preparations
    6. Good manufacturing practice for preparation of medicines
      1. Characteristics of the environment
      2. Staff
      3. Raw material
      4. Preparation of medicines in Pharmacy
    7. Cosmetics products
  12. Self-monitoring and HACCP: food infections - The microbiological quality control and the environment hygiene measures
    1. HACCP system
    2. Description of the HACCP system
      1. Preliminary phases of the application system
    3. Main features of HACCP system
      1. Principles 1 to 7
    4. Food microbiology
      1. Meaning of microorganisms in food
      2. Unfermented foods
        1. Meat, poultry, eggs, seafood, vegetables and fruit
      3. Fermented foods
        1. Fermented Vegetables, bread, cheese, fermented milk, fermented meat products
    5. HACCP in pharmacies according to Legislative Decree 193/07 and s.m.i

Notes on guidelines for the activity of sterilization as a collective protection against biological agents for the operator in health facilities (D.Lgs. 81/2008 e s.m.i.)

  1. The sterilization process and its enterprise management
  2. People involved
  3. Structural and technological requirements.
    1. Large / medium-sized hospitals
    2. Small hospitals
  4. Operating protocols
    1. Harvesting
    2. Decontamination
    3. Washing (manual, ultrasound, automated)
    4. Personal protective equipment [gloves, protective clothing, respiratory protection devices, for face protection (face shield or equivalent)]
  5. Rinsing, drying, inspection and maintenance
  6. Medical paper, envelopes and rolls in paper-laminated polymeric film, a polymeric material composition of various types usable in sheets, polyolefin and similar materials suitable for use in rolls or tubular, reusable Material: container
  7. Sterilization (steam sterilization, with ethylene oxide, with other physical methods, gas plasma of hydrogen peroxide, peracetic acid solutions
  8. Controls on environments and the process steps


Textbook Information

Prescott et Al. ‐ Microbiologia 7/ed. McGraw‐Hill – Vol. 1, 2, 3
Microbiologia Farmaceutica II ed, Edises




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