ONCOLOGIA MOLECOLARE E IMMUNOPATOLOGIA APPLICATE ALLE BIOTECNOLOGIE UMANE

12 CFU - 2° Semester

Teaching Staff

MASSIMO LIBRA - Module ONCOLOGIA MOLECOLARE APPLICATA ALLE BIOTECNOLOGIE UMANE - MED/04 - 6 CFU
PAOLO FABRIZIO FAGONE - Module IMMUNOLOGY TECHNIQUES APPLIED TO HUMAN BIOTECHNOLOGY - MED/46 - 3 CFU
LUCIA MALAGUARNERA - Module IMMUNOPATOLOGIA APPLICATA ALLE BIOTECNOLOGIE UMANE - MED/04 - 3 CFU

Learning Objectives

IMMUNOLOGY TECHNIQUES APPLIED TO HUMAN BIOTECHNOLOGY
The specific objectives of the course are to allow the student to acquire the fundamental notions on the diagnostic methods for the evaluation of immune system pathologies
IMMUNOPATOLOGIA APPLICATA ALLE BIOTECNOLOGIE UMANE
The course of General Pathology aims to provide the knowledge: of the extrinsic and intrinsic causes of human disease, interpreting their fundamental pathogenetic mechanisms; of the fundamental biological mechanisms of defense, damage response and reparation; of cellular alterations and of non-differentiated functions; primary alterations of differentiated functions of organs, systems and systems. It is also proposed to provide a cellular and molecular view of oncology. The mechanisms that regulate the normal, but also the "abnormal", the functioning of the immune system will be studied in depth. In particular the main objectives of the course of Immunology will be finalized to the knowledge: of the general characteristics of the antigens, of the molecular structures (antibodies, receptors, cytokines), of the cells, tissues and organs that make up the immune system; of the mechanisms of maturation of T and B lymphocytes and of regulation of central and peripheral tolerance: of the components and molecular mechanisms of innate and adaptive immune responses and of their cooperation and integration. Furthermore, the mechanisms underlying the alterations of the immune system will be investigated, in particular: hypersensitivity reactions, immunodeficiencies and autoimmune diseases.

Course Structure

IMMUNOLOGY TECHNIQUES APPLIED TO HUMAN BIOTECHNOLOGY
Polyclonal and monoclonal antibodies. Production techniques: Hybridomas and Phage Display

ELISA

Flow cytometric: principles and applications

Immunofluorescence: principles and applications in the diagnosis of autoimmune diseases

Sierology: principles and applications

Transplant immunology
IMMUNOPATOLOGIA APPLICATA ALLE BIOTECNOLOGIE UMANE
The course is divided into lectures and theoretical-practical lessons (exercises practical / demonstrative, seminar discussions).

Detailed Course Content

IMMUNOLOGY TECHNIQUES APPLIED TO HUMAN BIOTECHNOLOGY
Polyclonal and monoclonal antibodies. Production techniques: Hybridomas and Phage Display

ELISA: direct, indirect, sandwich and competitive ELISAs; Standard curve and interpolation of unknown values

Flow cytometric techniques: basics; compensation; cell cycle study; analysis of apoptosis

Immunofluorescence: principles and applications in the diagnosis of autoimmune diseases

Sierology: principles and applications

Transplant immunology

Textbook Information

IMMUNOLOGY TECHNIQUES APPLIED TO HUMAN BIOTECHNOLOGY
Abbas, Lichtman, Pillai. Immunologia cellulare e molecolare. Edra; 9° edizione 2018

Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019

Open in PDF format Versione in italiano