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METODOLOGIE DIAGNOSTICHE DI ANATOMIA PATOLOGICA

Teaching Staff

ROSARIO CALTABIANO - Module Sistematic Anatomic Pathology - MED/08 - 3 CFU
LIDIA PUZZO - Module Diagnostic Cytopathology - MED/08 - 2 CFU
GAETANO GIUSEPPE MAGRO - Module Immunohistochemistry and Molecular Pathology - MED/08 - 2 CFU

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Learning Objectives

• Sistematic Anatomic Pathology
  

  Knowledge of the morphological and molecular features, nosological classification and differential diagnosis of the main diseases. Knowledge of early diagnosis, prognostic and predictive factors of neoplastic diseases.

• Diagnostic Cytopathology
  

  At the end of the course the student will have to know the main types of diagnostic cytopathology exams, be able to set up and color the cytological preparations, know the basic principles of reactive and neoplastic cellular pathology; it must also be able to apply the general concepts of cytopathology to the main types of cytological samples.

• Immunohistochemistry and Molecular Pathology
  

  Knowledge of the main immunohistochemical and biomolecular techniques.

Course Structure

• Sistematic Anatomic Pathology
  

  Frontal teaching.

   

  Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
  

• Diagnostic Cytopathology
  

  Biweekly.

  Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
  

• Immunohistochemistry and Molecular Pathology
  

  Frontal teaching.

  Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
  

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Detailed Course Content

• Sistematic Anatomic Pathology
  

  Cellular lesions: hypertrophy, hyperplasia, metaplasia, dysplasia, carcinoma in situ, invasive carcinoma.

  Metastasis: cellular mechanisms and metastatic pathways (lymphatic, hematic, transperitoneal).

  Classification and pathological basis of malignant tumors: carcinomas, leukemia / lymphomas, sarcomas, neuroendocrine tumors, melanomas, brain tumors.

  Anatomopathological bases of the most common carcinomas: lung, breast, thyroid, gastric, colo-rectal, hepatic, renal, prostatic, bladder carcinoma.

  Anatomopathological diagnostics: cytological examinations (exfoliative cytology, faaspirative cytology); biopsy (endoscopy, needle biopsy, incisional biopsy and excisional biopsy); extemporaneous examination at the freezer.

  Staging of malignant neoplasms (TNM).

• Diagnostic Cytopathology
  

  Diagnostic cytopathology: advantages and limitations

  The different cytologic samples: needle and aspiration cytology

  Technique of cytological preparations: smear, thin, cell-block, cytocentrifugation

  Fixation and histochemical staining of cytological samples

  Techniques of immunocytochemistry, flow cytometry and FISH in diagnostic cytology

  Evaluation of cytological samples: criteria of adequacy

  Evaluation of morphological characteristics of the cells: normal cells, hyperplastic, atypical cells

  False negatives and false positives

  Diagnostic Cytopathology of thyroid

  Diagnostic Cytopathology of the lung

  Diagnostic cytopathology of the female genital tract: PAP tests and breast cytology

  Diagnostic Cytopathology of the urinary tract

  Diagnostic Cytopathology of the effusions

• Immunohistochemistry and Molecular Pathology
  

  Enzymatic histochemistry (special stains: eg Alcian-PAS, Giemsa, Rosso Congo, etc.)

  immunohistochemistry: unmasking, detection systems, antigen-antibody reaction, primary and secondary antibodies

  Main molecular biology techniques (FISH, CISH)

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Textbook Information

• Sistematic Anatomic Pathology
  

  Ruco-Scarpa Anatomia Patologica – Le Basi UTET

• Diagnostic Cytopathology
  

  Anatomia Patologica- Le basi- Luigi Ruco, Aldo Scarpa. Edizioni UTET

• Immunohistochemistry and Molecular Pathology
  

  Ruco Scarpa- Anatomia Patologica- Le basi, UTET

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