The specific training objectives of the Laboratory Diagnostic Methodologies Course are to enable students to acquire the basic notions for critical evaluation and interpretation of the results of the main diagnostic and biomolecular techniques used in clinical practice and basic research
The teaching will be carried out through the provision of classroom teaching lessons plus laboratory exercises to be carried out in the laboratories of the Biological Tower in order to improve the theoretical and practical knowledge acquired during the hours spent in the classroom
First generation sequencing: Sanger and Pyrosequencing
Microarray of gene expression
New generation sequencing NGS - Illumina and Roche 454 technology
Digital PCR - Droplet Digital PCR
Consultation of scientific literature and design of primers for PCR
1) Widmann: Interpretazione clinica degli esami di laboratorio. Ronald A. Sacher e Richard A. McPherson. 2001 Undicesima edizione. Mc Graw Hill libri Italia (Milano)
2) Giorgio Federici: Medicina di Laboratorio. 2008 Terza edizione. Mc Graw Hill libri Italia (Milano)