BIO/13 - 6 CFU - 2° Semester

Teaching Staff

Email: dipietro@unict.it
Office: Comparto 10, Edificio C, Via S. Sofia
Phone: 0953782075
Office Hours: Dal lunedì al Venerdi 11.00 - 13.00, previa prenotazione via email

Learning Objectives

Knowledge of the main techniques for the diagnosis of genetic diseases through DNA and RNA analysis (coding and non-coding). Understand the relationship between basic research and clinical applications and use the acquired technologies for the theoretical resolution of specific biological problems.

Course Structure

The course includes lectures and laboratory exercises. In particular, the students will learn to use online databases to search for nucleotide sequences of mRNA and non-coding RNA and programs for their analysis (sequence alignment, search for targets for microRNAs, pathways analysis, search for primers for PCR). Moreover, they will learn the main techniques of RNA extraction (from cells and biological fluids) and the Reverse Transcriptase Polymerase Chain Reaction (RT_PCR) Real Time.

Detailed Course Content

Monogenic diseases and complex phenotypes

Nucleic acid analysis

DNA – RNA extraction

Nucleic Acid hybridization

PCR, RT-PCR, Real Time PCR

Mutation detection of genetic disorders

High Throughput technologies

Textbook Information

1) Strachan e Read Genetica umana molecolare Zanichelli

2) Brown Biotecnologie Molecolari Zanichelli

3) Kreuzer e Massey Biologia Molecolare e Biotecnologie Zanichelli

4) Scientific literature

Open in PDF format Versione in italiano