The course is designed to provide students with a good foundation of knowledge in the terminology related to environmental pollution, to provide the foundation for understanding the reasons, the effects and the costs and let their learn the techniques of analysis, direct and indirect, of pollutants in air, water and soil.
As part of the course the introductory topics will be tackled to consolidate both the basic knowledge, which those relating to the theoretical and methodological bases
The specific training objectives of the Laboratory Diagnostic Methodologies Course are to enable students to acquire the basic notions for critical evaluation and interpretation of the results of the main diagnostic and biomolecular techniques used in clinical practice and basic research.
Direct lectures, Slides shown during teaching activities distributed at the end of the course.
The teaching will be carried out through the provision of classroom teaching lessons plus laboratory exercises to be carried out in the laboratories of the Biological Tower in order to improve the theoretical and practical knowledge acquired during the hours spent in the classroom.
Type of chemical pollutants: organic and inorganic products: pesticides and fertilizers.
1. First generation sequencing: Sanger and Pyrosequencing method.
2. Microarray of gene expression.
3. New generation sequencing NGS - Illumina technology pt 1/2
4. New generation NGS sequencing - Illumina pt 2/2 and Roche 454 technology
5. Droplet Digital PCR.
6. Role of the Microbiota and its implications in oncological and neuropsychiatric pathologies
7. Consultation of scientific literature and design of primers for PCR.
Chimica ambientale di Colin Baird, Michael Cann, Zanichelli. ISBN: 880817378X
1) Widmann: Interpretazione clinica degli esami di laboratorio. Ronald A. Sacher e Richard A. McPherson. 2001 Undicesima edizione. Mc Graw Hill libri Italia (Milano)
2) Giorgio Federici: Medicina di Laboratorio. 2008 Terza edizione. Mc Graw Hill libri Italia (Milano)